{"paper":{"title":"Rapid Single-Cell Measurement of Transient Transmembrane Water Flow under Osmotic Gradient","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"Guiren Wang, Hong Jiang, Jinnawat Jongkhumkrong, Qian Wang, Y.J. Chao","submitted_at":"2025-07-31T18:56:57Z","abstract_excerpt":"While aquaporin (AQP) gating dynamically regulates transmembrane water permeability for cellular homeostasis, its mechanisms remain poorly understood compared to ion channels. A central challenge is the lack of methods to measure water flow through AQPs with the spatiotemporal resolution and sensitivity equivalent to patch-clamp recordings of ion fluxes, a limitation stemming from the electrically silent nature of water transport. We introduce a technique to rapidly detect cytoplasmic flows induced by osmotic-gradient-driven transmembrane water transport in single adherent human cancer cells. "},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2508.00104","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2508.00104/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}