{"paper":{"title":"Building and maintaining a System of Intracellular Compartments","license":"http://creativecommons.org/licenses/by/4.0/","headline":"Vesicular transport and cisternal progression are two dynamical phases of one nonequilibrium fusion-fission process.","cross_cats":["nlin.AO","physics.bio-ph","q-bio.SC"],"primary_cat":"cond-mat.soft","authors_text":"Amit Kumar, Madan Rao","submitted_at":"2026-04-14T16:20:03Z","abstract_excerpt":"Organelle patterning and its heritability remain central mysteries in cell biology, highlighting the fundamental tension between genetic inheritance and self-assembly. Here, we explore the nonequilibrium assembly and emdedded size control of the Golgi cisternae and endosomes, amid a continuous flux of membrane traffic, within a stochastic framework of mechanochemical fusion-fission cycles that violate detailed balance. Using a dynamical systems approach, we identify distinct, robust regimes, ranging from fixed points to limit cycles with definite phase relations between cisternae. We identify "},"claims":{"count":4,"items":[{"kind":"strongest_claim","text":"Our analysis reveals that the two competing models of Golgi organization-vesicular transport and cisternal progression - are, in fact, two phases of the same underlying nonequilibrium process.","source":"verdict.strongest_claim","status":"machine_extracted","claim_id":"C1","attestation":"unclaimed"},{"kind":"weakest_assumption","text":"That the complex intracellular membrane dynamics can be adequately captured by a stochastic mechanochemical fusion-fission model violating detailed balance, with the resulting dynamical regimes directly mapping to biological phenotypes without additional molecular details.","source":"verdict.weakest_assumption","status":"machine_extracted","claim_id":"C2","attestation":"unclaimed"},{"kind":"one_line_summary","text":"A nonequilibrium dynamical systems model shows vesicular transport and cisternal progression as two phases of the same fusion-fission process governing organelle patterning and maintenance.","source":"verdict.one_line_summary","status":"machine_extracted","claim_id":"C3","attestation":"unclaimed"},{"kind":"headline","text":"Vesicular transport and cisternal progression are two dynamical phases of one nonequilibrium fusion-fission process.","source":"verdict.pith_extraction.headline","status":"machine_extracted","claim_id":"C4","attestation":"unclaimed"}],"snapshot_sha256":"a52a83768829858802e0df305283e5396b53979787485765b5e0338986dc14be"},"source":{"id":"2604.12930","kind":"arxiv","version":2},"verdict":{"id":"0bc44018-6139-4230-9a9a-19ea0c403461","model_set":{"reader":"grok-4.3"},"created_at":"2026-05-10T13:49:15.011795Z","strongest_claim":"Our analysis reveals that the two competing models of Golgi organization-vesicular transport and cisternal progression - are, in fact, two phases of the same underlying nonequilibrium process.","one_line_summary":"A nonequilibrium dynamical systems model shows vesicular transport and cisternal progression as two phases of the same fusion-fission process governing organelle patterning and maintenance.","pipeline_version":"pith-pipeline@v0.9.0","weakest_assumption":"That the complex intracellular membrane dynamics can be adequately captured by a stochastic mechanochemical fusion-fission model violating detailed balance, with the resulting dynamical regimes directly mapping to biological phenotypes without additional molecular details.","pith_extraction_headline":"Vesicular transport and cisternal progression are two dynamical phases of one nonequilibrium fusion-fission process."},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2604.12930/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}