{"paper":{"title":"Two-photon light-sheet live imaging at kilohertz frame rate using birefringence-based pulse splitting","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":["physics.app-ph","physics.bio-ph"],"primary_cat":"physics.optics","authors_text":"Antoine Hubert, Dale Gottlieb, Emmanuel Beaurepaire, Fr\\'ed\\'eric Druon, Lei Zhu, Pierre Mahou, Vincent Maioli, Willy Supatto","submitted_at":"2025-12-19T16:53:05Z","abstract_excerpt":"Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz), multi-point parallelized schemes have been proposed. Two-photon (2P) light-sheet microscopy emerges as an effective approach for high-speed multiphoton imaging of live specimens, as it enables parallelized excitation while minimizing the required laser power. However, optimizing the signal-to-photodamage ratio in 2P light-sheet microscopy requires to precisely con"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2512.17783","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2512.17783/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}