{"paper":{"title":"Polymorphic gene conferring susceptibility to insulin-dependent diabetes mellitus typed by ps-resolved FRET on nonamplified genomic DNA","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.bio-ph"],"primary_cat":"q-bio.GN","authors_text":"Alessandra Andreoni, Giovanna Tosi, Luca Nardo, Maria Bondani, Roberto S. Accolla","submitted_at":"2011-12-12T14:38:44Z","abstract_excerpt":"This work concerns the identification of the allelic sequences of the DQB1 gene of the human leukocyte antigen system conferring susceptibility to the development of insulin-dependent diabetes mellitus (IDDM) in DNA samples with no need of PCR amplification. Our method is based on the time-resolved analysis of a F\\\"orster energy-transfer mechanism that occurs in a dual-labeled fluorescent probe specific for the base sequence of the allelic variant of interest. Such an oligonucleotide probe is labeled, at the two ends, by a pair of chromophores that operate as donor and acceptor in a F\\\"orster "},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1112.2563","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}