{"paper":{"title":"Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":["cond-mat.soft"],"primary_cat":"physics.bio-ph","authors_text":"Alexey Zozulya, Anastasia Ragulskaya, Anders Madsen, Angel Rodriguez-Fernandez, Anita Girelli, Christian Gutt, Fajun Zhang, Fivos Perakis, Frank Schreiber, Hanna-Friederike Poggemann, Johannes M\\\"oller, J\\\"org Hallmann, Lisa Randolph, Maddalena Bin, Mariia Filianina, Mario Reiser, Marjorie Ladd-Parada, Markus Scholz, Mohammad Sayed Akhundzadeh, Nafisa Begam, Robert Rosca, Robert Schaffer, Roman Shayduk, Sharon Berkowicz, Sonja Timmermann, Sudipta Das, Tilo Seydel, Ulrike Boesenberg, Yuriy Chushkin","submitted_at":"2022-02-22T13:38:57Z","abstract_excerpt":"X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights into structural dynamics of biological macromolecule solutions. However, very high dose rates can lead to beam-induced dynamics and structural changes due to radiation damage. Here, we probe the dynamics of dense antibody protein (Ig-PEG) solutions using megahertz X-ray photon correlation spectroscopy (MHz-XPCS) at the European XFEL. By varying the total dose and dose rate, we identify a regime for measuring the motion of proteins in their first coordination shell, quantify XFEL-induced effects such as"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2202.10889","kind":"arxiv","version":2},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2202.10889/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}