{"paper":{"title":"Automated multi-dataset INST $^{13}$C metabolic flux analysis at microliter scale reveals robust fluxes but variable metabolite pools in $Corynebacterium~glutamicum$","license":"http://creativecommons.org/licenses/by-sa/4.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.QM","authors_text":"Anton Stratmann, Jochen Nie{\\ss}er, Katharina N\\\"oh, Martin Bey{\\ss}, Stephan Noack, Wolfgang Wiechert","submitted_at":"2026-05-24T16:27:40Z","abstract_excerpt":"Isotopically non-stationary metabolic flux analysis (INST $^{13}$C-MFA) provides unique insights into cellular physiology but is typically limited by low throughput and high experimental costs. Here, we present a miniaturized and automated workflow that integrates transient isotope labeling experiments with advanced computational modeling to enable parallel INST $^{13}$C-MFA at microliter scale. The approach is demonstrated for an evolved $Corynebacterium~glutamicum$ strain capable of efficient growth on ethanol, a substrate for which isotopically stationary $^{13}$C-MFA is inherently limited "},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2605.25155","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2605.25155/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"}