{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2018:2JZ74B2D5LK6MHXZOVQGIGCEUJ","short_pith_number":"pith:2JZ74B2D","canonical_record":{"source":{"id":"1811.05288","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"q-bio.PE","submitted_at":"2018-11-13T13:54:48Z","cross_cats_sorted":[],"title_canon_sha256":"637fc8015b0ec24782127cfb2322dca5b9f1236b935af02d115bad17a4542f25","abstract_canon_sha256":"ac2aaa93204b17a899f122c4fd3c3577d532f82a84cad5549fbf293cc9adaa1e"},"schema_version":"1.0"},"canonical_sha256":"d273fe0743ead5e61ef97560641844a2696091f2c80f42a9723b0c0ad1448d44","source":{"kind":"arxiv","id":"1811.05288","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1811.05288","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"arxiv_version","alias_value":"1811.05288v1","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1811.05288","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"pith_short_12","alias_value":"2JZ74B2D5LK6","created_at":"2026-05-18T12:32:02Z"},{"alias_kind":"pith_short_16","alias_value":"2JZ74B2D5LK6MHXZ","created_at":"2026-05-18T12:32:02Z"},{"alias_kind":"pith_short_8","alias_value":"2JZ74B2D","created_at":"2026-05-18T12:32:02Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2018:2JZ74B2D5LK6MHXZOVQGIGCEUJ","target":"record","payload":{"canonical_record":{"source":{"id":"1811.05288","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"q-bio.PE","submitted_at":"2018-11-13T13:54:48Z","cross_cats_sorted":[],"title_canon_sha256":"637fc8015b0ec24782127cfb2322dca5b9f1236b935af02d115bad17a4542f25","abstract_canon_sha256":"ac2aaa93204b17a899f122c4fd3c3577d532f82a84cad5549fbf293cc9adaa1e"},"schema_version":"1.0"},"canonical_sha256":"d273fe0743ead5e61ef97560641844a2696091f2c80f42a9723b0c0ad1448d44","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T00:00:46.433280Z","signature_b64":"KDhbuSmn2WlvabV7stjtaa3/2eh+q8EblSM7vKnBYN16o3mdnGuS2JFAQ4itlxwSLHD2iyxQ9xvPYv4cx3zjDA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"d273fe0743ead5e61ef97560641844a2696091f2c80f42a9723b0c0ad1448d44","last_reissued_at":"2026-05-18T00:00:46.432886Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T00:00:46.432886Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1811.05288","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T00:00:46Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Mq7GH/P+pRaYq5xbjgzVsE1denkkxzN7l3Y6iarnVf0LLlINjQlo600bckf7FuACkdlUGqTs5U7TFddiTikfDQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-21T10:36:45.651519Z"},"content_sha256":"51a0e0824b7199585f95370679aa5564513ceb00c199da72da6cf9df0bf6bbd3","schema_version":"1.0","event_id":"sha256:51a0e0824b7199585f95370679aa5564513ceb00c199da72da6cf9df0bf6bbd3"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2018:2JZ74B2D5LK6MHXZOVQGIGCEUJ","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Quantifying the performance of high-throughput directed evolution protocols","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.PE","authors_text":"Ad\\`ele Dram\\'e-Maign\\'e, Anton Zadorin, Iaroslava Golovkova, Yannick Rondelez","submitted_at":"2018-11-13T13:54:48Z","abstract_excerpt":"Most protocols for the high-throughput directed evolution of enzymes rely on random encapsulation to link phenotype and genotype. In order to optimize these approaches, or compare one to another, one needs a measure of their performance at extracting the best variants. We introduce here a new metric named the Selection Quality Index (SQI), which can be computed from a simple mock experiment with a known initial fraction of active variants. As opposed to previous approaches, our index integrates the random co-encapsulation of entities in compartments and comes with a straightforward experimenta"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1811.05288","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T00:00:46Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"OTv/Mv5gZ5hNUQVo5b+ZnMOrTi0AC3tcFbDh8pth7oQVtbth5df4V7+8tLuHQRX2D+ILgFyBipZGxB85V507AQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-21T10:36:45.651857Z"},"content_sha256":"febb44658846e923e1e295095982f112396938fedaec2dba1975c4653137623e","schema_version":"1.0","event_id":"sha256:febb44658846e923e1e295095982f112396938fedaec2dba1975c4653137623e"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/bundle.json","state_url":"https://pith.science/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-21T10:36:45Z","links":{"resolver":"https://pith.science/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ","bundle":"https://pith.science/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/bundle.json","state":"https://pith.science/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/state.json","well_known_bundle":"https://pith.science/.well-known/pith/2JZ74B2D5LK6MHXZOVQGIGCEUJ/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2018:2JZ74B2D5LK6MHXZOVQGIGCEUJ","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"ac2aaa93204b17a899f122c4fd3c3577d532f82a84cad5549fbf293cc9adaa1e","cross_cats_sorted":[],"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"q-bio.PE","submitted_at":"2018-11-13T13:54:48Z","title_canon_sha256":"637fc8015b0ec24782127cfb2322dca5b9f1236b935af02d115bad17a4542f25"},"schema_version":"1.0","source":{"id":"1811.05288","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1811.05288","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"arxiv_version","alias_value":"1811.05288v1","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1811.05288","created_at":"2026-05-18T00:00:46Z"},{"alias_kind":"pith_short_12","alias_value":"2JZ74B2D5LK6","created_at":"2026-05-18T12:32:02Z"},{"alias_kind":"pith_short_16","alias_value":"2JZ74B2D5LK6MHXZ","created_at":"2026-05-18T12:32:02Z"},{"alias_kind":"pith_short_8","alias_value":"2JZ74B2D","created_at":"2026-05-18T12:32:02Z"}],"graph_snapshots":[{"event_id":"sha256:febb44658846e923e1e295095982f112396938fedaec2dba1975c4653137623e","target":"graph","created_at":"2026-05-18T00:00:46Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Most protocols for the high-throughput directed evolution of enzymes rely on random encapsulation to link phenotype and genotype. In order to optimize these approaches, or compare one to another, one needs a measure of their performance at extracting the best variants. We introduce here a new metric named the Selection Quality Index (SQI), which can be computed from a simple mock experiment with a known initial fraction of active variants. As opposed to previous approaches, our index integrates the random co-encapsulation of entities in compartments and comes with a straightforward experimenta","authors_text":"Ad\\`ele Dram\\'e-Maign\\'e, Anton Zadorin, Iaroslava Golovkova, Yannick Rondelez","cross_cats":[],"headline":"","license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"q-bio.PE","submitted_at":"2018-11-13T13:54:48Z","title":"Quantifying the performance of high-throughput directed evolution protocols"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1811.05288","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:51a0e0824b7199585f95370679aa5564513ceb00c199da72da6cf9df0bf6bbd3","target":"record","created_at":"2026-05-18T00:00:46Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"ac2aaa93204b17a899f122c4fd3c3577d532f82a84cad5549fbf293cc9adaa1e","cross_cats_sorted":[],"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"q-bio.PE","submitted_at":"2018-11-13T13:54:48Z","title_canon_sha256":"637fc8015b0ec24782127cfb2322dca5b9f1236b935af02d115bad17a4542f25"},"schema_version":"1.0","source":{"id":"1811.05288","kind":"arxiv","version":1}},"canonical_sha256":"d273fe0743ead5e61ef97560641844a2696091f2c80f42a9723b0c0ad1448d44","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"d273fe0743ead5e61ef97560641844a2696091f2c80f42a9723b0c0ad1448d44","first_computed_at":"2026-05-18T00:00:46.432886Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T00:00:46.432886Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"KDhbuSmn2WlvabV7stjtaa3/2eh+q8EblSM7vKnBYN16o3mdnGuS2JFAQ4itlxwSLHD2iyxQ9xvPYv4cx3zjDA==","signature_status":"signed_v1","signed_at":"2026-05-18T00:00:46.433280Z","signed_message":"canonical_sha256_bytes"},"source_id":"1811.05288","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:51a0e0824b7199585f95370679aa5564513ceb00c199da72da6cf9df0bf6bbd3","sha256:febb44658846e923e1e295095982f112396938fedaec2dba1975c4653137623e"],"state_sha256":"39149d0738554be3cea65bfc02c42a43edef83a0f075a19079947bb62caf189a"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"cONfzGYUQyWBrQm579VWbZkviLDs0s+xiSBO2VPRd2AMNq8DIOhZ6tpG2+ncILGQaUEZQfdQdWj9PC7wLY4xCg==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-21T10:36:45.653626Z","bundle_sha256":"17adca61cf4db51d7dc6b1b08a824fa9069ed3b5eff7592292ca1939883830aa"}}