{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2025:2UHH46RG7ZOAQNRMGQEFODHXZ2","short_pith_number":"pith:2UHH46RG","canonical_record":{"source":{"id":"2506.22719","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"q-bio.BM","submitted_at":"2025-06-28T01:59:59Z","cross_cats_sorted":[],"title_canon_sha256":"e3b18b432c7e5dff3d70e7cd104ded5f993c369eaa8bb3b13e4066b209d8138e","abstract_canon_sha256":"41c53eabb3585dab373f36466f9e36dbf757f516de4f7147fa7ab8d8e20390ac"},"schema_version":"1.0"},"canonical_sha256":"d50e7e7a26fe5c08362c3408570cf7ceb0a3988b76a7ddcb8bf703f2af6ad385","source":{"kind":"arxiv","id":"2506.22719","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2506.22719","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"arxiv_version","alias_value":"2506.22719v1","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2506.22719","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_12","alias_value":"2UHH46RG7ZOA","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_16","alias_value":"2UHH46RG7ZOAQNRM","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_8","alias_value":"2UHH46RG","created_at":"2026-07-05T11:28:44Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2025:2UHH46RG7ZOAQNRMGQEFODHXZ2","target":"record","payload":{"canonical_record":{"source":{"id":"2506.22719","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"q-bio.BM","submitted_at":"2025-06-28T01:59:59Z","cross_cats_sorted":[],"title_canon_sha256":"e3b18b432c7e5dff3d70e7cd104ded5f993c369eaa8bb3b13e4066b209d8138e","abstract_canon_sha256":"41c53eabb3585dab373f36466f9e36dbf757f516de4f7147fa7ab8d8e20390ac"},"schema_version":"1.0"},"canonical_sha256":"d50e7e7a26fe5c08362c3408570cf7ceb0a3988b76a7ddcb8bf703f2af6ad385","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T11:28:44.141907Z","signature_b64":"YHbDJXHmuIds+gauiVezVxX45v3phzCy8jaA7BC2jqZuY6X5mBoi8D22nflUin6MobdllOZ4PZmweCs3zG65Dw==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"d50e7e7a26fe5c08362c3408570cf7ceb0a3988b76a7ddcb8bf703f2af6ad385","last_reissued_at":"2026-07-05T11:28:44.141391Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T11:28:44.141391Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"2506.22719","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T11:28:44Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Ka8fV2oZTG2f1eOgJ/vsF6BKKHqINmT0CsxR7taLZ3AM1LkNDlfPftpetYdep0c2CRkUIRGbTyD6WglM41TZBQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-04T06:56:06.698310Z"},"content_sha256":"5cd05f98cd55ad90bff2e484ddae3146194744ecf5d0ab4dcc15cdd0070e9612","schema_version":"1.0","event_id":"sha256:5cd05f98cd55ad90bff2e484ddae3146194744ecf5d0ab4dcc15cdd0070e9612"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2025:2UHH46RG7ZOAQNRMGQEFODHXZ2","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Resolving structural dynamics in situ through cryogenic electron tomography","license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.BM","authors_text":"Jackson Carrion, Joseph H. Davis","submitted_at":"2025-06-28T01:59:59Z","abstract_excerpt":"Cryo-electron tomography (cryo-ET) has emerged as a powerful tool for studying the structural heterogeneity of proteins and their complexes, offering insights into macromolecular dynamics directly within cells. Driven by recent computational advances, including powerful machine learning frameworks, researchers can now resolve both discrete structural states and continuous conformational changes from 3D subtomograms and stacks of 2D particle-images acquired across tilt-series. In this review, we survey recent innovations in particle classification and heterogeneous 3D reconstruction methods, fo"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2506.22719","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2506.22719/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T11:28:44Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"qv1qscvOlPv54eUtHD9ErlIC9DCBWN/p9lzCvxqrtga6LRkyoHhuEn8Jd7/Kb3yyIzevuCnDV55xhW/vITWfDA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-04T06:56:06.698870Z"},"content_sha256":"cea0d7d8ba7b47eb0aff08dbc6582a94076e55e4aa53d9421bc5d25fb2c9ebe4","schema_version":"1.0","event_id":"sha256:cea0d7d8ba7b47eb0aff08dbc6582a94076e55e4aa53d9421bc5d25fb2c9ebe4"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/bundle.json","state_url":"https://pith.science/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-08-04T06:56:06Z","links":{"resolver":"https://pith.science/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2","bundle":"https://pith.science/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/bundle.json","state":"https://pith.science/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/state.json","well_known_bundle":"https://pith.science/.well-known/pith/2UHH46RG7ZOAQNRMGQEFODHXZ2/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2025:2UHH46RG7ZOAQNRMGQEFODHXZ2","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"41c53eabb3585dab373f36466f9e36dbf757f516de4f7147fa7ab8d8e20390ac","cross_cats_sorted":[],"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"q-bio.BM","submitted_at":"2025-06-28T01:59:59Z","title_canon_sha256":"e3b18b432c7e5dff3d70e7cd104ded5f993c369eaa8bb3b13e4066b209d8138e"},"schema_version":"1.0","source":{"id":"2506.22719","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2506.22719","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"arxiv_version","alias_value":"2506.22719v1","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2506.22719","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_12","alias_value":"2UHH46RG7ZOA","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_16","alias_value":"2UHH46RG7ZOAQNRM","created_at":"2026-07-05T11:28:44Z"},{"alias_kind":"pith_short_8","alias_value":"2UHH46RG","created_at":"2026-07-05T11:28:44Z"}],"graph_snapshots":[{"event_id":"sha256:cea0d7d8ba7b47eb0aff08dbc6582a94076e55e4aa53d9421bc5d25fb2c9ebe4","target":"graph","created_at":"2026-07-05T11:28:44Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"integrity":{"available":true,"clean":true,"detectors_run":[],"endpoint":"/pith/2506.22719/integrity.json","findings":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938","summary":{"advisory":0,"by_detector":{},"critical":0,"informational":0}},"paper":{"abstract_excerpt":"Cryo-electron tomography (cryo-ET) has emerged as a powerful tool for studying the structural heterogeneity of proteins and their complexes, offering insights into macromolecular dynamics directly within cells. Driven by recent computational advances, including powerful machine learning frameworks, researchers can now resolve both discrete structural states and continuous conformational changes from 3D subtomograms and stacks of 2D particle-images acquired across tilt-series. In this review, we survey recent innovations in particle classification and heterogeneous 3D reconstruction methods, fo","authors_text":"Jackson Carrion, Joseph H. Davis","cross_cats":[],"headline":"","license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"q-bio.BM","submitted_at":"2025-06-28T01:59:59Z","title":"Resolving structural dynamics in situ through cryogenic electron tomography"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2506.22719","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:5cd05f98cd55ad90bff2e484ddae3146194744ecf5d0ab4dcc15cdd0070e9612","target":"record","created_at":"2026-07-05T11:28:44Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"41c53eabb3585dab373f36466f9e36dbf757f516de4f7147fa7ab8d8e20390ac","cross_cats_sorted":[],"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"q-bio.BM","submitted_at":"2025-06-28T01:59:59Z","title_canon_sha256":"e3b18b432c7e5dff3d70e7cd104ded5f993c369eaa8bb3b13e4066b209d8138e"},"schema_version":"1.0","source":{"id":"2506.22719","kind":"arxiv","version":1}},"canonical_sha256":"d50e7e7a26fe5c08362c3408570cf7ceb0a3988b76a7ddcb8bf703f2af6ad385","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"d50e7e7a26fe5c08362c3408570cf7ceb0a3988b76a7ddcb8bf703f2af6ad385","first_computed_at":"2026-07-05T11:28:44.141391Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-07-05T11:28:44.141391Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"YHbDJXHmuIds+gauiVezVxX45v3phzCy8jaA7BC2jqZuY6X5mBoi8D22nflUin6MobdllOZ4PZmweCs3zG65Dw==","signature_status":"signed_v1","signed_at":"2026-07-05T11:28:44.141907Z","signed_message":"canonical_sha256_bytes"},"source_id":"2506.22719","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:5cd05f98cd55ad90bff2e484ddae3146194744ecf5d0ab4dcc15cdd0070e9612","sha256:cea0d7d8ba7b47eb0aff08dbc6582a94076e55e4aa53d9421bc5d25fb2c9ebe4"],"state_sha256":"9dde56957524724769c6c4e530caeaf9a0ac65ee344c0572663c93ce2c08eb97"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Q3A6mZRmK0T2z323/BPKrRIoGrjUkHdtoNb0Eijc/V7s3fde7ksV5r7O1p8qoQJPhvfunNCjXp7Q6EeD8toODw==","signed_message":"bundle_sha256_bytes","signed_at":"2026-08-04T06:56:06.703371Z","bundle_sha256":"55e60d1f5007ca8220c8c89a58fb87de25803c1f9385b4791fe9992777808bbf"}}