{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2015:34WRVHCM4AUMOONSNUAGNH7YJX","short_pith_number":"pith:34WRVHCM","canonical_record":{"source":{"id":"1511.07977","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2015-11-25T07:30:07Z","cross_cats_sorted":[],"title_canon_sha256":"a41fa8f985af828913a2e633e1fb12e34b639c05e0366006a63e09cc93b97926","abstract_canon_sha256":"5262bc16f9b0c41c3e37a20535245e3ef654a2e38f423ccc1494cedc26f703a0"},"schema_version":"1.0"},"canonical_sha256":"df2d1a9c4ce028c739b26d00669ff84dfef40d4b496e86cb2947ca5003d87a1f","source":{"kind":"arxiv","id":"1511.07977","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1511.07977","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"arxiv_version","alias_value":"1511.07977v1","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1511.07977","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"pith_short_12","alias_value":"34WRVHCM4AUM","created_at":"2026-05-18T12:29:02Z"},{"alias_kind":"pith_short_16","alias_value":"34WRVHCM4AUMOONS","created_at":"2026-05-18T12:29:02Z"},{"alias_kind":"pith_short_8","alias_value":"34WRVHCM","created_at":"2026-05-18T12:29:02Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2015:34WRVHCM4AUMOONSNUAGNH7YJX","target":"record","payload":{"canonical_record":{"source":{"id":"1511.07977","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2015-11-25T07:30:07Z","cross_cats_sorted":[],"title_canon_sha256":"a41fa8f985af828913a2e633e1fb12e34b639c05e0366006a63e09cc93b97926","abstract_canon_sha256":"5262bc16f9b0c41c3e37a20535245e3ef654a2e38f423ccc1494cedc26f703a0"},"schema_version":"1.0"},"canonical_sha256":"df2d1a9c4ce028c739b26d00669ff84dfef40d4b496e86cb2947ca5003d87a1f","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T01:26:02.071307Z","signature_b64":"SUuDZVgrEHKUhGlj3ZeHpTvRCyVm/J2lCR8BAg008QuTeFQvn2Ajtg0VPodo3o/bZgs0w++I3fsY37BptjnyAg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"df2d1a9c4ce028c739b26d00669ff84dfef40d4b496e86cb2947ca5003d87a1f","last_reissued_at":"2026-05-18T01:26:02.070786Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T01:26:02.070786Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1511.07977","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T01:26:02Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Wt9lGu/B6YFcO5DLbvzcf3qHL5RpH1kx9J4ZNN191UPGyFDx8nZ8c8Alw7P+k5dp07lk3d0WZ9iSunHrAZpPAg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-05-27T13:30:37.224153Z"},"content_sha256":"98bada5f85e878c74f7aee2b1edd1add8eae63c75810f8d304ac877410a3ec30","schema_version":"1.0","event_id":"sha256:98bada5f85e878c74f7aee2b1edd1add8eae63c75810f8d304ac877410a3ec30"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2015:34WRVHCM4AUMOONSNUAGNH7YJX","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Importance of method validation: Implications of non-correlated observables in sweet taste receptor studies","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.BM","authors_text":"Manojendu Choudhury, Rani P Venkitakrishnan","submitted_at":"2015-11-25T07:30:07Z","abstract_excerpt":"Validation of research methodology is critical in research design. Correlation between experimental observables must be established before undertaking extensive experiments or propose mechanisms. This article shows that, observables in the popular calcium flux strength assay used in the characterization of sweetener-sweet taste receptor (STR) interaction are uncorrelated. In pursuit to find potential sweeteners and enhancers, calcium flux generated via G-protein coupling for wildtype and mutant receptors expressed on cell surface is measured to identify and localize sweetener binding sites. Re"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1511.07977","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T01:26:02Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Ng2iSUBELrCb5ixXOnuIaloS0n/lBJu0QFXnfU6FiiWgftEc3Eu39nqx8pYa9Nfei4xemJgSR1njJGbnWxVlCQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-05-27T13:30:37.224848Z"},"content_sha256":"91bff073f71206edefb9d848fcbdf04b2a5df2378779fbfb13a2b76ae248df6d","schema_version":"1.0","event_id":"sha256:91bff073f71206edefb9d848fcbdf04b2a5df2378779fbfb13a2b76ae248df6d"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/34WRVHCM4AUMOONSNUAGNH7YJX/bundle.json","state_url":"https://pith.science/pith/34WRVHCM4AUMOONSNUAGNH7YJX/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/34WRVHCM4AUMOONSNUAGNH7YJX/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-05-27T13:30:37Z","links":{"resolver":"https://pith.science/pith/34WRVHCM4AUMOONSNUAGNH7YJX","bundle":"https://pith.science/pith/34WRVHCM4AUMOONSNUAGNH7YJX/bundle.json","state":"https://pith.science/pith/34WRVHCM4AUMOONSNUAGNH7YJX/state.json","well_known_bundle":"https://pith.science/.well-known/pith/34WRVHCM4AUMOONSNUAGNH7YJX/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2015:34WRVHCM4AUMOONSNUAGNH7YJX","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"5262bc16f9b0c41c3e37a20535245e3ef654a2e38f423ccc1494cedc26f703a0","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2015-11-25T07:30:07Z","title_canon_sha256":"a41fa8f985af828913a2e633e1fb12e34b639c05e0366006a63e09cc93b97926"},"schema_version":"1.0","source":{"id":"1511.07977","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1511.07977","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"arxiv_version","alias_value":"1511.07977v1","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1511.07977","created_at":"2026-05-18T01:26:02Z"},{"alias_kind":"pith_short_12","alias_value":"34WRVHCM4AUM","created_at":"2026-05-18T12:29:02Z"},{"alias_kind":"pith_short_16","alias_value":"34WRVHCM4AUMOONS","created_at":"2026-05-18T12:29:02Z"},{"alias_kind":"pith_short_8","alias_value":"34WRVHCM","created_at":"2026-05-18T12:29:02Z"}],"graph_snapshots":[{"event_id":"sha256:91bff073f71206edefb9d848fcbdf04b2a5df2378779fbfb13a2b76ae248df6d","target":"graph","created_at":"2026-05-18T01:26:02Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Validation of research methodology is critical in research design. Correlation between experimental observables must be established before undertaking extensive experiments or propose mechanisms. This article shows that, observables in the popular calcium flux strength assay used in the characterization of sweetener-sweet taste receptor (STR) interaction are uncorrelated. In pursuit to find potential sweeteners and enhancers, calcium flux generated via G-protein coupling for wildtype and mutant receptors expressed on cell surface is measured to identify and localize sweetener binding sites. Re","authors_text":"Manojendu Choudhury, Rani P Venkitakrishnan","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2015-11-25T07:30:07Z","title":"Importance of method validation: Implications of non-correlated observables in sweet taste receptor studies"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1511.07977","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:98bada5f85e878c74f7aee2b1edd1add8eae63c75810f8d304ac877410a3ec30","target":"record","created_at":"2026-05-18T01:26:02Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"5262bc16f9b0c41c3e37a20535245e3ef654a2e38f423ccc1494cedc26f703a0","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2015-11-25T07:30:07Z","title_canon_sha256":"a41fa8f985af828913a2e633e1fb12e34b639c05e0366006a63e09cc93b97926"},"schema_version":"1.0","source":{"id":"1511.07977","kind":"arxiv","version":1}},"canonical_sha256":"df2d1a9c4ce028c739b26d00669ff84dfef40d4b496e86cb2947ca5003d87a1f","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"df2d1a9c4ce028c739b26d00669ff84dfef40d4b496e86cb2947ca5003d87a1f","first_computed_at":"2026-05-18T01:26:02.070786Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T01:26:02.070786Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"SUuDZVgrEHKUhGlj3ZeHpTvRCyVm/J2lCR8BAg008QuTeFQvn2Ajtg0VPodo3o/bZgs0w++I3fsY37BptjnyAg==","signature_status":"signed_v1","signed_at":"2026-05-18T01:26:02.071307Z","signed_message":"canonical_sha256_bytes"},"source_id":"1511.07977","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:98bada5f85e878c74f7aee2b1edd1add8eae63c75810f8d304ac877410a3ec30","sha256:91bff073f71206edefb9d848fcbdf04b2a5df2378779fbfb13a2b76ae248df6d"],"state_sha256":"85596b840bf08df3f02c954cb2577c1fb8760d3e610d5f96aeb48e552fa671c3"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"4EK/I4RnF2glDqC4cybVh8KTVhswHp06VdcT8KeI+/AyMQbS9X9+cr85oitIQvL7rCTQ+VlbxmfgrMOX8h7FCQ==","signed_message":"bundle_sha256_bytes","signed_at":"2026-05-27T13:30:37.228402Z","bundle_sha256":"abd65f7e717e6ef70e4c3d1847fb86fc0f17e9d64bb4566e91c5b6f89e05d5cb"}}