{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2016:6JBXNQTFN6RH33CPTBGSUMR6ON","short_pith_number":"pith:6JBXNQTF","schema_version":"1.0","canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","source":{"kind":"arxiv","id":"1605.05804","version":1},"attestation_state":"computed","paper":{"title":"Shear-Induced Nitric Oxide Production by Endothelial Cells","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["q-bio.CB"],"primary_cat":"q-bio.MN","authors_text":"Daniel M. Tartakovsky, Justin G. Laughlin, Krishna Sriram, Padmini Rangamani","submitted_at":"2016-05-19T04:08:19Z","abstract_excerpt":"We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts. The first is rapid activation of calcium, which results in formation of calcium-calmodulin complexes, followed by recruitment of eNOS from caveolae. The second is phosphoryaltion of eNOS by protein kinases PKC and AKT. The model also includes a negative feedback loo"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1605.05804","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","cross_cats_sorted":["q-bio.CB"],"title_canon_sha256":"0d0fe5b4021806d770e8535e615660cf0e413f95294f9613893275047b969ad4","abstract_canon_sha256":"7e7397d8ef321d62f405691b312abfe6887eac631655059761d60ffe81afa186"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T01:11:07.935883Z","signature_b64":"kcktPIHMnRW/OdphbC8bGVoQ2plokMBGq8yE/9iWrXg8+c5htegMfrgt5CCnwR2OKX/OAqgYBsdmfpSqfmVzCQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","last_reissued_at":"2026-05-18T01:11:07.935300Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T01:11:07.935300Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Shear-Induced Nitric Oxide Production by Endothelial Cells","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["q-bio.CB"],"primary_cat":"q-bio.MN","authors_text":"Daniel M. Tartakovsky, Justin G. Laughlin, Krishna Sriram, Padmini Rangamani","submitted_at":"2016-05-19T04:08:19Z","abstract_excerpt":"We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts. The first is rapid activation of calcium, which results in formation of calcium-calmodulin complexes, followed by recruitment of eNOS from caveolae. The second is phosphoryaltion of eNOS by protein kinases PKC and AKT. The model also includes a negative feedback loo"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1605.05804","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1605.05804","created_at":"2026-05-18T01:11:07.935391+00:00"},{"alias_kind":"arxiv_version","alias_value":"1605.05804v1","created_at":"2026-05-18T01:11:07.935391+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1605.05804","created_at":"2026-05-18T01:11:07.935391+00:00"},{"alias_kind":"pith_short_12","alias_value":"6JBXNQTFN6RH","created_at":"2026-05-18T12:30:01.593930+00:00"},{"alias_kind":"pith_short_16","alias_value":"6JBXNQTFN6RH33CP","created_at":"2026-05-18T12:30:01.593930+00:00"},{"alias_kind":"pith_short_8","alias_value":"6JBXNQTF","created_at":"2026-05-18T12:30:01.593930+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON","json":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON.json","graph_json":"https://pith.science/api/pith-number/6JBXNQTFN6RH33CPTBGSUMR6ON/graph.json","events_json":"https://pith.science/api/pith-number/6JBXNQTFN6RH33CPTBGSUMR6ON/events.json","paper":"https://pith.science/paper/6JBXNQTF"},"agent_actions":{"view_html":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON","download_json":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON.json","view_paper":"https://pith.science/paper/6JBXNQTF","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1605.05804&json=true","fetch_graph":"https://pith.science/api/pith-number/6JBXNQTFN6RH33CPTBGSUMR6ON/graph.json","fetch_events":"https://pith.science/api/pith-number/6JBXNQTFN6RH33CPTBGSUMR6ON/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/action/timestamp_anchor","attest_storage":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/action/storage_attestation","attest_author":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/action/author_attestation","sign_citation":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/action/citation_signature","submit_replication":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/action/replication_record"}},"created_at":"2026-05-18T01:11:07.935391+00:00","updated_at":"2026-05-18T01:11:07.935391+00:00"}