{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2016:6JBXNQTFN6RH33CPTBGSUMR6ON","short_pith_number":"pith:6JBXNQTF","canonical_record":{"source":{"id":"1605.05804","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","cross_cats_sorted":["q-bio.CB"],"title_canon_sha256":"0d0fe5b4021806d770e8535e615660cf0e413f95294f9613893275047b969ad4","abstract_canon_sha256":"7e7397d8ef321d62f405691b312abfe6887eac631655059761d60ffe81afa186"},"schema_version":"1.0"},"canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","source":{"kind":"arxiv","id":"1605.05804","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1605.05804","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"arxiv_version","alias_value":"1605.05804v1","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1605.05804","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"pith_short_12","alias_value":"6JBXNQTFN6RH","created_at":"2026-05-18T12:30:01Z"},{"alias_kind":"pith_short_16","alias_value":"6JBXNQTFN6RH33CP","created_at":"2026-05-18T12:30:01Z"},{"alias_kind":"pith_short_8","alias_value":"6JBXNQTF","created_at":"2026-05-18T12:30:01Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2016:6JBXNQTFN6RH33CPTBGSUMR6ON","target":"record","payload":{"canonical_record":{"source":{"id":"1605.05804","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","cross_cats_sorted":["q-bio.CB"],"title_canon_sha256":"0d0fe5b4021806d770e8535e615660cf0e413f95294f9613893275047b969ad4","abstract_canon_sha256":"7e7397d8ef321d62f405691b312abfe6887eac631655059761d60ffe81afa186"},"schema_version":"1.0"},"canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T01:11:07.935883Z","signature_b64":"kcktPIHMnRW/OdphbC8bGVoQ2plokMBGq8yE/9iWrXg8+c5htegMfrgt5CCnwR2OKX/OAqgYBsdmfpSqfmVzCQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","last_reissued_at":"2026-05-18T01:11:07.935300Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T01:11:07.935300Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1605.05804","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T01:11:07Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"+5vkw8QdvQqRUe8m7vcBv6s2KrjMGLP45Uf8jXhh1RnujiBWN1z9RvOsP3IZDVajd3u3UMkCXZ7I4KHHWoaADg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-03T18:21:46.224961Z"},"content_sha256":"9ec9c1400c44867a8a60fb73222c0918cc0ef01fe56f2127dbe340ae2ddcb1fe","schema_version":"1.0","event_id":"sha256:9ec9c1400c44867a8a60fb73222c0918cc0ef01fe56f2127dbe340ae2ddcb1fe"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2016:6JBXNQTFN6RH33CPTBGSUMR6ON","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Shear-Induced Nitric Oxide Production by Endothelial Cells","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["q-bio.CB"],"primary_cat":"q-bio.MN","authors_text":"Daniel M. Tartakovsky, Justin G. Laughlin, Krishna Sriram, Padmini Rangamani","submitted_at":"2016-05-19T04:08:19Z","abstract_excerpt":"We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts. The first is rapid activation of calcium, which results in formation of calcium-calmodulin complexes, followed by recruitment of eNOS from caveolae. The second is phosphoryaltion of eNOS by protein kinases PKC and AKT. The model also includes a negative feedback loo"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1605.05804","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T01:11:07Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"kK2y3pOVUPEW70AipX2DRQj0swQLV9Uhou/hBAL6Im23dBMtD4QYGoIe0g8vIuWFzjTT7JG0o5ykybGu/GiSCw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-03T18:21:46.225328Z"},"content_sha256":"537e36c74e6bd6d7f01c72f15ebaa98f225fc99afc80a55f1e88c9b9cf6b5a0d","schema_version":"1.0","event_id":"sha256:537e36c74e6bd6d7f01c72f15ebaa98f225fc99afc80a55f1e88c9b9cf6b5a0d"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/bundle.json","state_url":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-03T18:21:46Z","links":{"resolver":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON","bundle":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/bundle.json","state":"https://pith.science/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/state.json","well_known_bundle":"https://pith.science/.well-known/pith/6JBXNQTFN6RH33CPTBGSUMR6ON/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2016:6JBXNQTFN6RH33CPTBGSUMR6ON","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"7e7397d8ef321d62f405691b312abfe6887eac631655059761d60ffe81afa186","cross_cats_sorted":["q-bio.CB"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","title_canon_sha256":"0d0fe5b4021806d770e8535e615660cf0e413f95294f9613893275047b969ad4"},"schema_version":"1.0","source":{"id":"1605.05804","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1605.05804","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"arxiv_version","alias_value":"1605.05804v1","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1605.05804","created_at":"2026-05-18T01:11:07Z"},{"alias_kind":"pith_short_12","alias_value":"6JBXNQTFN6RH","created_at":"2026-05-18T12:30:01Z"},{"alias_kind":"pith_short_16","alias_value":"6JBXNQTFN6RH33CP","created_at":"2026-05-18T12:30:01Z"},{"alias_kind":"pith_short_8","alias_value":"6JBXNQTF","created_at":"2026-05-18T12:30:01Z"}],"graph_snapshots":[{"event_id":"sha256:537e36c74e6bd6d7f01c72f15ebaa98f225fc99afc80a55f1e88c9b9cf6b5a0d","target":"graph","created_at":"2026-05-18T01:11:07Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts. The first is rapid activation of calcium, which results in formation of calcium-calmodulin complexes, followed by recruitment of eNOS from caveolae. The second is phosphoryaltion of eNOS by protein kinases PKC and AKT. The model also includes a negative feedback loo","authors_text":"Daniel M. Tartakovsky, Justin G. Laughlin, Krishna Sriram, Padmini Rangamani","cross_cats":["q-bio.CB"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","title":"Shear-Induced Nitric Oxide Production by Endothelial Cells"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1605.05804","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:9ec9c1400c44867a8a60fb73222c0918cc0ef01fe56f2127dbe340ae2ddcb1fe","target":"record","created_at":"2026-05-18T01:11:07Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"7e7397d8ef321d62f405691b312abfe6887eac631655059761d60ffe81afa186","cross_cats_sorted":["q-bio.CB"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.MN","submitted_at":"2016-05-19T04:08:19Z","title_canon_sha256":"0d0fe5b4021806d770e8535e615660cf0e413f95294f9613893275047b969ad4"},"schema_version":"1.0","source":{"id":"1605.05804","kind":"arxiv","version":1}},"canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"f24376c2656fa27dec4f984d2a323e735a3b7b4bf0e9d4973f81d24fa2a622c8","first_computed_at":"2026-05-18T01:11:07.935300Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T01:11:07.935300Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"kcktPIHMnRW/OdphbC8bGVoQ2plokMBGq8yE/9iWrXg8+c5htegMfrgt5CCnwR2OKX/OAqgYBsdmfpSqfmVzCQ==","signature_status":"signed_v1","signed_at":"2026-05-18T01:11:07.935883Z","signed_message":"canonical_sha256_bytes"},"source_id":"1605.05804","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:9ec9c1400c44867a8a60fb73222c0918cc0ef01fe56f2127dbe340ae2ddcb1fe","sha256:537e36c74e6bd6d7f01c72f15ebaa98f225fc99afc80a55f1e88c9b9cf6b5a0d"],"state_sha256":"b92a8313f556cd5f17ad890b3d75774b41ce8321a842b9f51f318d5708ddbc71"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"cQuqWnmaH4eYDmlQfkcMcshPM0QZqvLW+cwAW21pjAyCBOW80QMzFOKAWLMiAauvbBx8qoISSjWtFxxt/ilwBw==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-03T18:21:46.227293Z","bundle_sha256":"358404ec551991a3b95adca2da25f5ab17fc052b361f30d51af3294e4490198c"}}