{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2015:BAHFJPFW3X5NOTVGZ2FVQPJILT","short_pith_number":"pith:BAHFJPFW","schema_version":"1.0","canonical_sha256":"080e54bcb6ddfad74ea6ce8b583d285cd1c542567b7533ba938d377b2d31bdac","source":{"kind":"arxiv","id":"1505.02683","version":1},"attestation_state":"computed","paper":{"title":"Imaging Extracellular Protein Concentration with Nanoplasmonic Sensors","license":"http://creativecommons.org/licenses/publicdomain/","headline":"","cross_cats":["physics.bio-ph"],"primary_cat":"q-bio.QM","authors_text":"Deepa Raghu, James B. Delehanty, Jeff M. Byers, Joseph A. Christodoulides, Marc P. Raphael","submitted_at":"2015-05-11T16:05:55Z","abstract_excerpt":"Extracellular protein concentrations and gradients queue a wide range of cellular responses, such as cell motility and division. Spatio-temporal quantification of these concentrations as produced by cells has proven challenging. As a result, artificial gradients must be introduced to the cell culture to correlate signal and response. Here we demonstrate a label-free nanoplasmonic imaging technique that can directly map protein concentrations as secreted by single cells in real time and which integrates with standard live-cell microscopes. When used to measure the secretion of antibodies from h"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1505.02683","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/publicdomain/","primary_cat":"q-bio.QM","submitted_at":"2015-05-11T16:05:55Z","cross_cats_sorted":["physics.bio-ph"],"title_canon_sha256":"21d18b3a03f41846ccd3de5486c4b30c86af8eea24989d2b787106901e7ea851","abstract_canon_sha256":"3edbede655212181f29e35b8c60defc30e994a75d4a0faee24f8f53fd1c2e953"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T02:16:11.870939Z","signature_b64":"7SLIMuRvO7EB7Sq+tzwKqKObxISEK7VtH5ikJnL1e3a9V586McU1aADl/1Jcy/tM5/aLRRrWiAWJ+5JF7dhEDQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"080e54bcb6ddfad74ea6ce8b583d285cd1c542567b7533ba938d377b2d31bdac","last_reissued_at":"2026-05-18T02:16:11.870395Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T02:16:11.870395Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Imaging Extracellular Protein Concentration with Nanoplasmonic Sensors","license":"http://creativecommons.org/licenses/publicdomain/","headline":"","cross_cats":["physics.bio-ph"],"primary_cat":"q-bio.QM","authors_text":"Deepa Raghu, James B. Delehanty, Jeff M. Byers, Joseph A. Christodoulides, Marc P. Raphael","submitted_at":"2015-05-11T16:05:55Z","abstract_excerpt":"Extracellular protein concentrations and gradients queue a wide range of cellular responses, such as cell motility and division. Spatio-temporal quantification of these concentrations as produced by cells has proven challenging. As a result, artificial gradients must be introduced to the cell culture to correlate signal and response. Here we demonstrate a label-free nanoplasmonic imaging technique that can directly map protein concentrations as secreted by single cells in real time and which integrates with standard live-cell microscopes. When used to measure the secretion of antibodies from h"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1505.02683","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1505.02683","created_at":"2026-05-18T02:16:11.870474+00:00"},{"alias_kind":"arxiv_version","alias_value":"1505.02683v1","created_at":"2026-05-18T02:16:11.870474+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1505.02683","created_at":"2026-05-18T02:16:11.870474+00:00"},{"alias_kind":"pith_short_12","alias_value":"BAHFJPFW3X5N","created_at":"2026-05-18T12:29:14.074870+00:00"},{"alias_kind":"pith_short_16","alias_value":"BAHFJPFW3X5NOTVG","created_at":"2026-05-18T12:29:14.074870+00:00"},{"alias_kind":"pith_short_8","alias_value":"BAHFJPFW","created_at":"2026-05-18T12:29:14.074870+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT","json":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT.json","graph_json":"https://pith.science/api/pith-number/BAHFJPFW3X5NOTVGZ2FVQPJILT/graph.json","events_json":"https://pith.science/api/pith-number/BAHFJPFW3X5NOTVGZ2FVQPJILT/events.json","paper":"https://pith.science/paper/BAHFJPFW"},"agent_actions":{"view_html":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT","download_json":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT.json","view_paper":"https://pith.science/paper/BAHFJPFW","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1505.02683&json=true","fetch_graph":"https://pith.science/api/pith-number/BAHFJPFW3X5NOTVGZ2FVQPJILT/graph.json","fetch_events":"https://pith.science/api/pith-number/BAHFJPFW3X5NOTVGZ2FVQPJILT/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT/action/timestamp_anchor","attest_storage":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT/action/storage_attestation","attest_author":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT/action/author_attestation","sign_citation":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT/action/citation_signature","submit_replication":"https://pith.science/pith/BAHFJPFW3X5NOTVGZ2FVQPJILT/action/replication_record"}},"created_at":"2026-05-18T02:16:11.870474+00:00","updated_at":"2026-05-18T02:16:11.870474+00:00"}