{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2012:BZYD5EJBDMTRDD6XIQVDSAJGSG","short_pith_number":"pith:BZYD5EJB","canonical_record":{"source":{"id":"1206.0858","kind":"arxiv","version":2},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2012-06-05T09:40:15Z","cross_cats_sorted":["physics.bio-ph","q-bio.BM"],"title_canon_sha256":"de16dc7d005d84aa1063d8b16ef514318039896aaf3dfda5996862422afd5c3f","abstract_canon_sha256":"b34bf51ee2ddba7a6afc0e734e762f4ab68d4e33f1e25c750a4b4d28cede3cbd"},"schema_version":"1.0"},"canonical_sha256":"0e703e91211b27118fd7442a39012691825d122ce21b2621a19616c702a3e2f9","source":{"kind":"arxiv","id":"1206.0858","version":2},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1206.0858","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"arxiv_version","alias_value":"1206.0858v2","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1206.0858","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"pith_short_12","alias_value":"BZYD5EJBDMTR","created_at":"2026-05-18T12:27:01Z"},{"alias_kind":"pith_short_16","alias_value":"BZYD5EJBDMTRDD6X","created_at":"2026-05-18T12:27:01Z"},{"alias_kind":"pith_short_8","alias_value":"BZYD5EJB","created_at":"2026-05-18T12:27:01Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2012:BZYD5EJBDMTRDD6XIQVDSAJGSG","target":"record","payload":{"canonical_record":{"source":{"id":"1206.0858","kind":"arxiv","version":2},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2012-06-05T09:40:15Z","cross_cats_sorted":["physics.bio-ph","q-bio.BM"],"title_canon_sha256":"de16dc7d005d84aa1063d8b16ef514318039896aaf3dfda5996862422afd5c3f","abstract_canon_sha256":"b34bf51ee2ddba7a6afc0e734e762f4ab68d4e33f1e25c750a4b4d28cede3cbd"},"schema_version":"1.0"},"canonical_sha256":"0e703e91211b27118fd7442a39012691825d122ce21b2621a19616c702a3e2f9","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:53:33.326565Z","signature_b64":"/t7Rwma1dSg0y939NUw9uHGsOKx+m2lEM+e4qjTaY/z+tX0JIFwsjDQmu0J3qfaltJo1hiEqj6sPjiKwJUrmBQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"0e703e91211b27118fd7442a39012691825d122ce21b2621a19616c702a3e2f9","last_reissued_at":"2026-05-18T03:53:33.325750Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:53:33.325750Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1206.0858","source_version":2,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:53:33Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"JIfzHWU4HI2ahZkrmvnE8pMVUJXUe8kjyKTsAvgnGgROnIq/0Y5Z0zDjMRcsC1j8WitUQWc0cEdRvHkPg0W2Cw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-05-28T23:41:18.198939Z"},"content_sha256":"d9cc4baaded8c3023cdf8d6f1ac8bfbd4df3db986ac59746710bfbc6ff9823bb","schema_version":"1.0","event_id":"sha256:d9cc4baaded8c3023cdf8d6f1ac8bfbd4df3db986ac59746710bfbc6ff9823bb"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2012:BZYD5EJBDMTRDD6XIQVDSAJGSG","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Origins of concentration dependence of waiting times for single-molecule fluorescence binding","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.bio-ph","q-bio.BM"],"primary_cat":"q-bio.QM","authors_text":"Jin Yang, John E. Pearson","submitted_at":"2012-06-05T09:40:15Z","abstract_excerpt":"Binary fluorescence time series obtained from single-molecule imaging experiments can be used to infer protein binding kinetics, in particular, association and dissociation rate constants from waiting time statistics of fluorescence intensity changes. In many cases, rate constants inferred from fluorescence time series exhibit nonintuitive dependence on ligand concentration. Here we examine several possible mechanistic and technical origins that may induce ligand dependence of rate constants. Using aggregated Markov models, we show under the condition of detailed balance that non-fluorescent b"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1206.0858","kind":"arxiv","version":2},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:53:33Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"nqdDvVvZBSdbm4he81GAXc1lgCAiAPSf3bs5yjfqTVHwfmDkKCDeDvfqWB2vQGrZDFeYIk5bfOhn6gMMUhQTCw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-05-28T23:41:18.199674Z"},"content_sha256":"ec77e55e8eedfb15e7075d2062a2e63dd67442ddb81d8c20cc829c0e4f5dd405","schema_version":"1.0","event_id":"sha256:ec77e55e8eedfb15e7075d2062a2e63dd67442ddb81d8c20cc829c0e4f5dd405"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/bundle.json","state_url":"https://pith.science/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-05-28T23:41:18Z","links":{"resolver":"https://pith.science/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG","bundle":"https://pith.science/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/bundle.json","state":"https://pith.science/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/state.json","well_known_bundle":"https://pith.science/.well-known/pith/BZYD5EJBDMTRDD6XIQVDSAJGSG/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2012:BZYD5EJBDMTRDD6XIQVDSAJGSG","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"b34bf51ee2ddba7a6afc0e734e762f4ab68d4e33f1e25c750a4b4d28cede3cbd","cross_cats_sorted":["physics.bio-ph","q-bio.BM"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2012-06-05T09:40:15Z","title_canon_sha256":"de16dc7d005d84aa1063d8b16ef514318039896aaf3dfda5996862422afd5c3f"},"schema_version":"1.0","source":{"id":"1206.0858","kind":"arxiv","version":2}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1206.0858","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"arxiv_version","alias_value":"1206.0858v2","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1206.0858","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"pith_short_12","alias_value":"BZYD5EJBDMTR","created_at":"2026-05-18T12:27:01Z"},{"alias_kind":"pith_short_16","alias_value":"BZYD5EJBDMTRDD6X","created_at":"2026-05-18T12:27:01Z"},{"alias_kind":"pith_short_8","alias_value":"BZYD5EJB","created_at":"2026-05-18T12:27:01Z"}],"graph_snapshots":[{"event_id":"sha256:ec77e55e8eedfb15e7075d2062a2e63dd67442ddb81d8c20cc829c0e4f5dd405","target":"graph","created_at":"2026-05-18T03:53:33Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Binary fluorescence time series obtained from single-molecule imaging experiments can be used to infer protein binding kinetics, in particular, association and dissociation rate constants from waiting time statistics of fluorescence intensity changes. In many cases, rate constants inferred from fluorescence time series exhibit nonintuitive dependence on ligand concentration. Here we examine several possible mechanistic and technical origins that may induce ligand dependence of rate constants. Using aggregated Markov models, we show under the condition of detailed balance that non-fluorescent b","authors_text":"Jin Yang, John E. Pearson","cross_cats":["physics.bio-ph","q-bio.BM"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2012-06-05T09:40:15Z","title":"Origins of concentration dependence of waiting times for single-molecule fluorescence binding"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1206.0858","kind":"arxiv","version":2},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:d9cc4baaded8c3023cdf8d6f1ac8bfbd4df3db986ac59746710bfbc6ff9823bb","target":"record","created_at":"2026-05-18T03:53:33Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"b34bf51ee2ddba7a6afc0e734e762f4ab68d4e33f1e25c750a4b4d28cede3cbd","cross_cats_sorted":["physics.bio-ph","q-bio.BM"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2012-06-05T09:40:15Z","title_canon_sha256":"de16dc7d005d84aa1063d8b16ef514318039896aaf3dfda5996862422afd5c3f"},"schema_version":"1.0","source":{"id":"1206.0858","kind":"arxiv","version":2}},"canonical_sha256":"0e703e91211b27118fd7442a39012691825d122ce21b2621a19616c702a3e2f9","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"0e703e91211b27118fd7442a39012691825d122ce21b2621a19616c702a3e2f9","first_computed_at":"2026-05-18T03:53:33.325750Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T03:53:33.325750Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"/t7Rwma1dSg0y939NUw9uHGsOKx+m2lEM+e4qjTaY/z+tX0JIFwsjDQmu0J3qfaltJo1hiEqj6sPjiKwJUrmBQ==","signature_status":"signed_v1","signed_at":"2026-05-18T03:53:33.326565Z","signed_message":"canonical_sha256_bytes"},"source_id":"1206.0858","source_kind":"arxiv","source_version":2}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:d9cc4baaded8c3023cdf8d6f1ac8bfbd4df3db986ac59746710bfbc6ff9823bb","sha256:ec77e55e8eedfb15e7075d2062a2e63dd67442ddb81d8c20cc829c0e4f5dd405"],"state_sha256":"1c2fd56523bfca56c06e14752bc369b2bc7a0bd9f832ebed6d2a345a1823757d"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"MOycWsQ3iiQXWCjbHSR/+PKIdT3cDkeCfeNL9eSgtqZKvdZnezcF1vO6q0uKYfbWJz49FhZ8rmkTGSaeUhJaCQ==","signed_message":"bundle_sha256_bytes","signed_at":"2026-05-28T23:41:18.203294Z","bundle_sha256":"53dd08bb7578e444b5d1ab36c040b4c651a1baff576e943a2ab827471c3c6be5"}}