{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2014:DENEEIHFMFRK7AFMJFDIIHSRRH","short_pith_number":"pith:DENEEIHF","canonical_record":{"source":{"id":"1411.7947","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2014-11-28T17:34:48Z","cross_cats_sorted":[],"title_canon_sha256":"527d784f07937fb03519aaeea4ce3cafb4fd7e096c2569fb6824b65317f7c806","abstract_canon_sha256":"a189083eb3480f9d3bd5d77489cd9bf93ea357df40caf7ca3114123f3ec67627"},"schema_version":"1.0"},"canonical_sha256":"191a4220e56162af80ac4946841e5189fb5269d05ed90c8e236f9da738720185","source":{"kind":"arxiv","id":"1411.7947","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1411.7947","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"arxiv_version","alias_value":"1411.7947v1","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1411.7947","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"pith_short_12","alias_value":"DENEEIHFMFRK","created_at":"2026-05-18T12:28:25Z"},{"alias_kind":"pith_short_16","alias_value":"DENEEIHFMFRK7AFM","created_at":"2026-05-18T12:28:25Z"},{"alias_kind":"pith_short_8","alias_value":"DENEEIHF","created_at":"2026-05-18T12:28:25Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2014:DENEEIHFMFRK7AFMJFDIIHSRRH","target":"record","payload":{"canonical_record":{"source":{"id":"1411.7947","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2014-11-28T17:34:48Z","cross_cats_sorted":[],"title_canon_sha256":"527d784f07937fb03519aaeea4ce3cafb4fd7e096c2569fb6824b65317f7c806","abstract_canon_sha256":"a189083eb3480f9d3bd5d77489cd9bf93ea357df40caf7ca3114123f3ec67627"},"schema_version":"1.0"},"canonical_sha256":"191a4220e56162af80ac4946841e5189fb5269d05ed90c8e236f9da738720185","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T02:32:31.974292Z","signature_b64":"3VWt8A218jihwybJ21kx8ZEmATy40yXSLkpFbj6XiqabBBHNXIkxs1sjvaxf0svtt4ru9UpE14uyRY+3GAgVDw==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"191a4220e56162af80ac4946841e5189fb5269d05ed90c8e236f9da738720185","last_reissued_at":"2026-05-18T02:32:31.973853Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T02:32:31.973853Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1411.7947","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:32:31Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"HjzDwe8Pp3Ll7CIqw9OKjF8X8ggzqcifkTBumGA7cO0KDo4+F7stzgoqBp5c9kbBujWhkKePhHPts8aSPJLlDg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-03T18:55:20.273409Z"},"content_sha256":"8e54fd3d1adf15fe2ced4ad9e47b1ee268421511899f4ee73df7acc2755c70a3","schema_version":"1.0","event_id":"sha256:8e54fd3d1adf15fe2ced4ad9e47b1ee268421511899f4ee73df7acc2755c70a3"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2014:DENEEIHFMFRK7AFMJFDIIHSRRH","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"F. T. Amaral, L. Siman, O. N. Mesquita, P. M. S. Roma, U. Agero","submitted_at":"2014-11-28T17:34:48Z","abstract_excerpt":"In this paper the bright-field defocusing microscopy (DM) technique is presented. DM is able to obtain quantitative information of each plane/surface of pure phase objects, as live unlabeled cells, and its application to red blood cells (RBCs) is demonstrated. Based on contrast, simple methods to obtain thickness profile and three dimensional (3D) total reconstruction of RBCs are proposed and the actual height profiles of upper and lower surface-membranes (lipid bilayer$/$cytoskeleton) of discocyte and stomatocyte red cells are presented as examples. In addition, using the mean square contrast"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1411.7947","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:32:31Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"wUXBp18AusFQGh1nkxzcEpvWVSLx19mvC40/EQOxG2Wc0xsERH7bkEBiY/NszjcIeDyPcQ8BoPsmVUmpHhlHAA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-03T18:55:20.273761Z"},"content_sha256":"e76faec7feeb381fb7effc94168c5537a1b29b3d1e91ce96cebfaed9ffd0600e","schema_version":"1.0","event_id":"sha256:e76faec7feeb381fb7effc94168c5537a1b29b3d1e91ce96cebfaed9ffd0600e"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/bundle.json","state_url":"https://pith.science/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-03T18:55:20Z","links":{"resolver":"https://pith.science/pith/DENEEIHFMFRK7AFMJFDIIHSRRH","bundle":"https://pith.science/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/bundle.json","state":"https://pith.science/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/state.json","well_known_bundle":"https://pith.science/.well-known/pith/DENEEIHFMFRK7AFMJFDIIHSRRH/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2014:DENEEIHFMFRK7AFMJFDIIHSRRH","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"a189083eb3480f9d3bd5d77489cd9bf93ea357df40caf7ca3114123f3ec67627","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2014-11-28T17:34:48Z","title_canon_sha256":"527d784f07937fb03519aaeea4ce3cafb4fd7e096c2569fb6824b65317f7c806"},"schema_version":"1.0","source":{"id":"1411.7947","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1411.7947","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"arxiv_version","alias_value":"1411.7947v1","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1411.7947","created_at":"2026-05-18T02:32:31Z"},{"alias_kind":"pith_short_12","alias_value":"DENEEIHFMFRK","created_at":"2026-05-18T12:28:25Z"},{"alias_kind":"pith_short_16","alias_value":"DENEEIHFMFRK7AFM","created_at":"2026-05-18T12:28:25Z"},{"alias_kind":"pith_short_8","alias_value":"DENEEIHF","created_at":"2026-05-18T12:28:25Z"}],"graph_snapshots":[{"event_id":"sha256:e76faec7feeb381fb7effc94168c5537a1b29b3d1e91ce96cebfaed9ffd0600e","target":"graph","created_at":"2026-05-18T02:32:31Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"In this paper the bright-field defocusing microscopy (DM) technique is presented. DM is able to obtain quantitative information of each plane/surface of pure phase objects, as live unlabeled cells, and its application to red blood cells (RBCs) is demonstrated. Based on contrast, simple methods to obtain thickness profile and three dimensional (3D) total reconstruction of RBCs are proposed and the actual height profiles of upper and lower surface-membranes (lipid bilayer$/$cytoskeleton) of discocyte and stomatocyte red cells are presented as examples. In addition, using the mean square contrast","authors_text":"F. T. Amaral, L. Siman, O. N. Mesquita, P. M. S. Roma, U. Agero","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2014-11-28T17:34:48Z","title":"Shape reconstruction and height fluctuations of red blood cells using defocusing microscopy"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1411.7947","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:8e54fd3d1adf15fe2ced4ad9e47b1ee268421511899f4ee73df7acc2755c70a3","target":"record","created_at":"2026-05-18T02:32:31Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"a189083eb3480f9d3bd5d77489cd9bf93ea357df40caf7ca3114123f3ec67627","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2014-11-28T17:34:48Z","title_canon_sha256":"527d784f07937fb03519aaeea4ce3cafb4fd7e096c2569fb6824b65317f7c806"},"schema_version":"1.0","source":{"id":"1411.7947","kind":"arxiv","version":1}},"canonical_sha256":"191a4220e56162af80ac4946841e5189fb5269d05ed90c8e236f9da738720185","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"191a4220e56162af80ac4946841e5189fb5269d05ed90c8e236f9da738720185","first_computed_at":"2026-05-18T02:32:31.973853Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T02:32:31.973853Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"3VWt8A218jihwybJ21kx8ZEmATy40yXSLkpFbj6XiqabBBHNXIkxs1sjvaxf0svtt4ru9UpE14uyRY+3GAgVDw==","signature_status":"signed_v1","signed_at":"2026-05-18T02:32:31.974292Z","signed_message":"canonical_sha256_bytes"},"source_id":"1411.7947","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:8e54fd3d1adf15fe2ced4ad9e47b1ee268421511899f4ee73df7acc2755c70a3","sha256:e76faec7feeb381fb7effc94168c5537a1b29b3d1e91ce96cebfaed9ffd0600e"],"state_sha256":"90499b6ec0d9695a2a986ad365b07cf78fb71b64bf96cd92bf451d0fa3e084fc"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"2CtF2ECkW8ijbyK6IL7mh6ay98FGOptxY0Eqwl/BCRhSbpmPVWEQ7z1o2JsWFFuUM9TyMbL8LkdxcsHUbB7ABA==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-03T18:55:20.276044Z","bundle_sha256":"7269f6b1bac55437a1f8c524d641b391f1fa90b1378cd50a85e19001b60a59d2"}}