{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2019:G3BUX24T3VAJGXQ34BSIHTYJUK","short_pith_number":"pith:G3BUX24T","canonical_record":{"source":{"id":"1912.06783","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2019-12-14T04:59:41Z","cross_cats_sorted":["physics.chem-ph"],"title_canon_sha256":"3a8c19cf17e52cd25584882f621f6f8f4dc1f762fb9eb3868662045b4db1d0ba","abstract_canon_sha256":"d4f0b9af8b23aa3fee273d2cfbbe3ae58e15d5fe13a6116816b897da4e8fa519"},"schema_version":"1.0"},"canonical_sha256":"36c34beb93dd40935e1be06483cf09a28c5669479daaaa89b46431fd8e0e2106","source":{"kind":"arxiv","id":"1912.06783","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1912.06783","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"arxiv_version","alias_value":"1912.06783v1","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1912.06783","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_12","alias_value":"G3BUX24T3VAJ","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_16","alias_value":"G3BUX24T3VAJGXQ3","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_8","alias_value":"G3BUX24T","created_at":"2026-07-05T01:48:25Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2019:G3BUX24T3VAJGXQ34BSIHTYJUK","target":"record","payload":{"canonical_record":{"source":{"id":"1912.06783","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2019-12-14T04:59:41Z","cross_cats_sorted":["physics.chem-ph"],"title_canon_sha256":"3a8c19cf17e52cd25584882f621f6f8f4dc1f762fb9eb3868662045b4db1d0ba","abstract_canon_sha256":"d4f0b9af8b23aa3fee273d2cfbbe3ae58e15d5fe13a6116816b897da4e8fa519"},"schema_version":"1.0"},"canonical_sha256":"36c34beb93dd40935e1be06483cf09a28c5669479daaaa89b46431fd8e0e2106","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T01:48:25.422015Z","signature_b64":"SLLE5ZG2Pngwff7CS0TNL4FNagPmfZKj1y9z4HjNlyDKSB07rz7Vyv+NPjHs7deA9poxTtw/fG61K3vrg9veAQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"36c34beb93dd40935e1be06483cf09a28c5669479daaaa89b46431fd8e0e2106","last_reissued_at":"2026-07-05T01:48:25.421599Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T01:48:25.421599Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1912.06783","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T01:48:25Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"DaS3czs8QZofTD6rXfRTNtFRtkgoCLBNLdDpLKsCVCTWzMXL8p+N8otF2m4isztqtnzgZmOCPoNUtuPDxIZbBA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-13T01:22:02.982783Z"},"content_sha256":"a2ee3b6544c9eebf4b10aaa84b07760e7a21615cdc8c013a9193642094f42bdc","schema_version":"1.0","event_id":"sha256:a2ee3b6544c9eebf4b10aaa84b07760e7a21615cdc8c013a9193642094f42bdc"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2019:G3BUX24T3VAJGXQ34BSIHTYJUK","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Depletion interactions modulate coupled folding and binding in crowded environments","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.chem-ph"],"primary_cat":"physics.bio-ph","authors_text":"Andrea Soranno, Benjamin Schuler, Daniel Nettels, Franziska Zosel","submitted_at":"2019-12-14T04:59:41Z","abstract_excerpt":"Intrinsically disordered proteins (IDPs) abound in cellular regulation. Their interactions are often transitory and highly sensitive to salt concentration and posttranslational modifications. However, little is known about the effect of macromolecular crowding on the kinetics and stability of the interactions of IDPs with their cellular targets. Here, we investigate the influence of crowding on the coupled folding and binding between two IDPs, using polyethylene glycol as a crowding agent across a broad size range. Single-molecule F\\\"orster resonance energy transfer allows us to quantify sever"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1912.06783","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/1912.06783/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T01:48:25Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"0DtOcQsPP/t18UPZW+CnJPqlUA4owknOMta/sNgM1ZshMn9RI83NjWbc5bwkPT3iKGJ181v+u4/vXSwNJD81BA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-13T01:22:02.983607Z"},"content_sha256":"a554f5c88f7e25df719b861b8b95e526dc4ab064fc84e7109721b32362fb56a3","schema_version":"1.0","event_id":"sha256:a554f5c88f7e25df719b861b8b95e526dc4ab064fc84e7109721b32362fb56a3"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/bundle.json","state_url":"https://pith.science/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-08-13T01:22:02Z","links":{"resolver":"https://pith.science/pith/G3BUX24T3VAJGXQ34BSIHTYJUK","bundle":"https://pith.science/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/bundle.json","state":"https://pith.science/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/state.json","well_known_bundle":"https://pith.science/.well-known/pith/G3BUX24T3VAJGXQ34BSIHTYJUK/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2019:G3BUX24T3VAJGXQ34BSIHTYJUK","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"d4f0b9af8b23aa3fee273d2cfbbe3ae58e15d5fe13a6116816b897da4e8fa519","cross_cats_sorted":["physics.chem-ph"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2019-12-14T04:59:41Z","title_canon_sha256":"3a8c19cf17e52cd25584882f621f6f8f4dc1f762fb9eb3868662045b4db1d0ba"},"schema_version":"1.0","source":{"id":"1912.06783","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1912.06783","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"arxiv_version","alias_value":"1912.06783v1","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1912.06783","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_12","alias_value":"G3BUX24T3VAJ","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_16","alias_value":"G3BUX24T3VAJGXQ3","created_at":"2026-07-05T01:48:25Z"},{"alias_kind":"pith_short_8","alias_value":"G3BUX24T","created_at":"2026-07-05T01:48:25Z"}],"graph_snapshots":[{"event_id":"sha256:a554f5c88f7e25df719b861b8b95e526dc4ab064fc84e7109721b32362fb56a3","target":"graph","created_at":"2026-07-05T01:48:25Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"integrity":{"available":true,"clean":true,"detectors_run":[],"endpoint":"/pith/1912.06783/integrity.json","findings":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938","summary":{"advisory":0,"by_detector":{},"critical":0,"informational":0}},"paper":{"abstract_excerpt":"Intrinsically disordered proteins (IDPs) abound in cellular regulation. Their interactions are often transitory and highly sensitive to salt concentration and posttranslational modifications. However, little is known about the effect of macromolecular crowding on the kinetics and stability of the interactions of IDPs with their cellular targets. Here, we investigate the influence of crowding on the coupled folding and binding between two IDPs, using polyethylene glycol as a crowding agent across a broad size range. Single-molecule F\\\"orster resonance energy transfer allows us to quantify sever","authors_text":"Andrea Soranno, Benjamin Schuler, Daniel Nettels, Franziska Zosel","cross_cats":["physics.chem-ph"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2019-12-14T04:59:41Z","title":"Depletion interactions modulate coupled folding and binding in crowded environments"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1912.06783","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:a2ee3b6544c9eebf4b10aaa84b07760e7a21615cdc8c013a9193642094f42bdc","target":"record","created_at":"2026-07-05T01:48:25Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"d4f0b9af8b23aa3fee273d2cfbbe3ae58e15d5fe13a6116816b897da4e8fa519","cross_cats_sorted":["physics.chem-ph"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2019-12-14T04:59:41Z","title_canon_sha256":"3a8c19cf17e52cd25584882f621f6f8f4dc1f762fb9eb3868662045b4db1d0ba"},"schema_version":"1.0","source":{"id":"1912.06783","kind":"arxiv","version":1}},"canonical_sha256":"36c34beb93dd40935e1be06483cf09a28c5669479daaaa89b46431fd8e0e2106","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"36c34beb93dd40935e1be06483cf09a28c5669479daaaa89b46431fd8e0e2106","first_computed_at":"2026-07-05T01:48:25.421599Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-07-05T01:48:25.421599Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"SLLE5ZG2Pngwff7CS0TNL4FNagPmfZKj1y9z4HjNlyDKSB07rz7Vyv+NPjHs7deA9poxTtw/fG61K3vrg9veAQ==","signature_status":"signed_v1","signed_at":"2026-07-05T01:48:25.422015Z","signed_message":"canonical_sha256_bytes"},"source_id":"1912.06783","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:a2ee3b6544c9eebf4b10aaa84b07760e7a21615cdc8c013a9193642094f42bdc","sha256:a554f5c88f7e25df719b861b8b95e526dc4ab064fc84e7109721b32362fb56a3"],"state_sha256":"ed511ed6d9514c7e458f0cde1fa70b8a4f98b751a227f847f6eebb3ae2b300d1"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"i17zDEKSeyraQ+oV/oNvNXVTLGP4ZEdZ2IXwmuwc9m0WlKIXhCgMapJ/QvtA1ArnFCXLRGKGpBzNcu5eRuxBBw==","signed_message":"bundle_sha256_bytes","signed_at":"2026-08-13T01:22:02.990468Z","bundle_sha256":"72db98ae6a2510df13297d1c3d85c7214f5a448999d6a7e42c6655ff7e9e829b"}}