{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2014:G3LQ6SMSOKTO2VBRPLO5HHMJWK","short_pith_number":"pith:G3LQ6SMS","canonical_record":{"source":{"id":"1402.4287","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2014-02-18T10:49:37Z","cross_cats_sorted":[],"title_canon_sha256":"f01ab13b07987392b32569c8721fad21a5dc2e1e36596f36e5a548c8af99b63e","abstract_canon_sha256":"3aa5e0950bb50c051eaa6a5d66765b41ec25c8fa64c060a298894a89d7f81709"},"schema_version":"1.0"},"canonical_sha256":"36d70f499272a6ed54317addd39d89b2a7b7a50a83eae2a97d9b83797227fe56","source":{"kind":"arxiv","id":"1402.4287","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1402.4287","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"arxiv_version","alias_value":"1402.4287v1","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1402.4287","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"pith_short_12","alias_value":"G3LQ6SMSOKTO","created_at":"2026-05-18T12:28:28Z"},{"alias_kind":"pith_short_16","alias_value":"G3LQ6SMSOKTO2VBR","created_at":"2026-05-18T12:28:28Z"},{"alias_kind":"pith_short_8","alias_value":"G3LQ6SMS","created_at":"2026-05-18T12:28:28Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2014:G3LQ6SMSOKTO2VBRPLO5HHMJWK","target":"record","payload":{"canonical_record":{"source":{"id":"1402.4287","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2014-02-18T10:49:37Z","cross_cats_sorted":[],"title_canon_sha256":"f01ab13b07987392b32569c8721fad21a5dc2e1e36596f36e5a548c8af99b63e","abstract_canon_sha256":"3aa5e0950bb50c051eaa6a5d66765b41ec25c8fa64c060a298894a89d7f81709"},"schema_version":"1.0"},"canonical_sha256":"36d70f499272a6ed54317addd39d89b2a7b7a50a83eae2a97d9b83797227fe56","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T02:58:44.268656Z","signature_b64":"UQnkvw4s6NmVoo2BgNlbiksnE8xEBgfWnSle72YbGxwQFFX/zyYfFJFvoaWnkuFCmi+fUt2tYXUHjCMhqejWCw==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"36d70f499272a6ed54317addd39d89b2a7b7a50a83eae2a97d9b83797227fe56","last_reissued_at":"2026-05-18T02:58:44.268046Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T02:58:44.268046Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1402.4287","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:58:44Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"uqpDdIlTqYi/7qJPJBLfPo7v2UA0XuenPvIqRYFFUjT1gxjf6GDI8qQx9oPrLYadxiSYbgzivuOpStbaWYXxCA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-12T00:29:10.877542Z"},"content_sha256":"2cade8524b8b66a688d15a9484e7e9eab2a4f2491476a679c15f06955232de75","schema_version":"1.0","event_id":"sha256:2cade8524b8b66a688d15a9484e7e9eab2a4f2491476a679c15f06955232de75"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2014:G3LQ6SMSOKTO2VBRPLO5HHMJWK","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Protonation States of Remote Residues Affect Binding-Release Dynamics of the Ligand but not the Conformation of apo Ferric Binding Protein","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.BM","authors_text":"Ali Rana Atilgan, Canan Atilgan, Gokce Guven","submitted_at":"2014-02-18T10:49:37Z","abstract_excerpt":"We have studied the apo (Fe3+ free) form of periplasmic ferric binding protein (FbpA) under different conditions and we have monitored the changes in the binding and release dynamics of H2PO4- that acts as a synergistic anion in the presence of Fe3+. Our simulations predict a dissociation constant of 2.2$\\pm$0.2 mM which is in remarkable agreement with the experimentally measured value of 2.3$\\pm$0.3 mM under the same ionization strength and pH conditions. We apply perturbations relevant for changes in environmental conditions as (i) different values of ionic strength (IS), and (ii) protonatio"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1402.4287","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:58:44Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"fIEiTfncVPPdKO5pF6NfEnZGBNSrGlBD8h2lZBaVBFCydzszf1TiZOHb1RsT8bLAgVxv8zX/qNhztOdW+811Bw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-12T00:29:10.877923Z"},"content_sha256":"95dbc07ea336d61a1e4e43b186f06472040f5c6e0538e284389c3e30cef3f1b4","schema_version":"1.0","event_id":"sha256:95dbc07ea336d61a1e4e43b186f06472040f5c6e0538e284389c3e30cef3f1b4"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/bundle.json","state_url":"https://pith.science/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-12T00:29:10Z","links":{"resolver":"https://pith.science/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK","bundle":"https://pith.science/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/bundle.json","state":"https://pith.science/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/state.json","well_known_bundle":"https://pith.science/.well-known/pith/G3LQ6SMSOKTO2VBRPLO5HHMJWK/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2014:G3LQ6SMSOKTO2VBRPLO5HHMJWK","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"3aa5e0950bb50c051eaa6a5d66765b41ec25c8fa64c060a298894a89d7f81709","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2014-02-18T10:49:37Z","title_canon_sha256":"f01ab13b07987392b32569c8721fad21a5dc2e1e36596f36e5a548c8af99b63e"},"schema_version":"1.0","source":{"id":"1402.4287","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1402.4287","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"arxiv_version","alias_value":"1402.4287v1","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1402.4287","created_at":"2026-05-18T02:58:44Z"},{"alias_kind":"pith_short_12","alias_value":"G3LQ6SMSOKTO","created_at":"2026-05-18T12:28:28Z"},{"alias_kind":"pith_short_16","alias_value":"G3LQ6SMSOKTO2VBR","created_at":"2026-05-18T12:28:28Z"},{"alias_kind":"pith_short_8","alias_value":"G3LQ6SMS","created_at":"2026-05-18T12:28:28Z"}],"graph_snapshots":[{"event_id":"sha256:95dbc07ea336d61a1e4e43b186f06472040f5c6e0538e284389c3e30cef3f1b4","target":"graph","created_at":"2026-05-18T02:58:44Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"We have studied the apo (Fe3+ free) form of periplasmic ferric binding protein (FbpA) under different conditions and we have monitored the changes in the binding and release dynamics of H2PO4- that acts as a synergistic anion in the presence of Fe3+. Our simulations predict a dissociation constant of 2.2$\\pm$0.2 mM which is in remarkable agreement with the experimentally measured value of 2.3$\\pm$0.3 mM under the same ionization strength and pH conditions. We apply perturbations relevant for changes in environmental conditions as (i) different values of ionic strength (IS), and (ii) protonatio","authors_text":"Ali Rana Atilgan, Canan Atilgan, Gokce Guven","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2014-02-18T10:49:37Z","title":"Protonation States of Remote Residues Affect Binding-Release Dynamics of the Ligand but not the Conformation of apo Ferric Binding Protein"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1402.4287","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:2cade8524b8b66a688d15a9484e7e9eab2a4f2491476a679c15f06955232de75","target":"record","created_at":"2026-05-18T02:58:44Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"3aa5e0950bb50c051eaa6a5d66765b41ec25c8fa64c060a298894a89d7f81709","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2014-02-18T10:49:37Z","title_canon_sha256":"f01ab13b07987392b32569c8721fad21a5dc2e1e36596f36e5a548c8af99b63e"},"schema_version":"1.0","source":{"id":"1402.4287","kind":"arxiv","version":1}},"canonical_sha256":"36d70f499272a6ed54317addd39d89b2a7b7a50a83eae2a97d9b83797227fe56","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"36d70f499272a6ed54317addd39d89b2a7b7a50a83eae2a97d9b83797227fe56","first_computed_at":"2026-05-18T02:58:44.268046Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T02:58:44.268046Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"UQnkvw4s6NmVoo2BgNlbiksnE8xEBgfWnSle72YbGxwQFFX/zyYfFJFvoaWnkuFCmi+fUt2tYXUHjCMhqejWCw==","signature_status":"signed_v1","signed_at":"2026-05-18T02:58:44.268656Z","signed_message":"canonical_sha256_bytes"},"source_id":"1402.4287","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:2cade8524b8b66a688d15a9484e7e9eab2a4f2491476a679c15f06955232de75","sha256:95dbc07ea336d61a1e4e43b186f06472040f5c6e0538e284389c3e30cef3f1b4"],"state_sha256":"db4b21e1728015554b9b4f12ae13a7507cc6d0feb71b9611a863cd0049119446"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"HJEt1Sj9jssbkZDq6SjOaw6yFjkQsDfExt6p478sMfVqWULdLf2KcfISRPC6eZqSvlkxke0NomCt3Ore12l+Dw==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-12T00:29:10.879949Z","bundle_sha256":"873a8d1c19568a516a95c480f8cd9d03b6c8d960b588811a33861997b778af31"}}