{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2025:HBGA4CS3G57W7IGXNEIDKUJKO4","short_pith_number":"pith:HBGA4CS3","schema_version":"1.0","canonical_sha256":"384c0e0a5b377f6fa0d7691035512a77002a7ddf8ab63de14250436ae8521b59","source":{"kind":"arxiv","id":"2507.13211","version":1},"attestation_state":"computed","paper":{"title":"The fantastic single-molecule techniques","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"Boyang Hua, Chenyue Kang, Gege Duan, Huang Tang, Kun Li, Shuting Liu, Xiang Wang, Xi Zhang, Zheng Li","submitted_at":"2025-07-17T15:19:12Z","abstract_excerpt":"In the past 40 years, single-molecule techniques have been rapidly developed and widely applied in numerous fields of biology researches, offering new insights that conventional biochemical assays cannot discover. In this review, to help fully appreciate the powerfulness of single-molecule methods, we systemically summarize the various advantages of performing biochemical assays at the single-molecule level. Inspired by these examples, we propose a new single-molecule polysome profiling technique, to demonstrate that this strategy is not limited to the few special \"outliers\". Finally, we point"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"2507.13211","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"physics.bio-ph","submitted_at":"2025-07-17T15:19:12Z","cross_cats_sorted":[],"title_canon_sha256":"68b246e9098b6535c2e9fb783857194b72ecf2bda98ff4be4fdfa5cb8e266fb8","abstract_canon_sha256":"45f516a9e1097d32782b16e9ab7218ba853c2c34efb8cf31dab8cca13ed3b7da"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T11:38:56.406420Z","signature_b64":"eH8jNxUODL8Xb4zPqEQpAgSBNv86O3Ij4EI00bP4rtijD5IIw8RfCYCEDEcpQPmhLBwaR6322A6VrPqWUpT0Dg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"384c0e0a5b377f6fa0d7691035512a77002a7ddf8ab63de14250436ae8521b59","last_reissued_at":"2026-07-05T11:38:56.406014Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T11:38:56.406014Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"The fantastic single-molecule techniques","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"Boyang Hua, Chenyue Kang, Gege Duan, Huang Tang, Kun Li, Shuting Liu, Xiang Wang, Xi Zhang, Zheng Li","submitted_at":"2025-07-17T15:19:12Z","abstract_excerpt":"In the past 40 years, single-molecule techniques have been rapidly developed and widely applied in numerous fields of biology researches, offering new insights that conventional biochemical assays cannot discover. In this review, to help fully appreciate the powerfulness of single-molecule methods, we systemically summarize the various advantages of performing biochemical assays at the single-molecule level. Inspired by these examples, we propose a new single-molecule polysome profiling technique, to demonstrate that this strategy is not limited to the few special \"outliers\". Finally, we point"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2507.13211","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2507.13211/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"2507.13211","created_at":"2026-07-05T11:38:56.406067+00:00"},{"alias_kind":"arxiv_version","alias_value":"2507.13211v1","created_at":"2026-07-05T11:38:56.406067+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2507.13211","created_at":"2026-07-05T11:38:56.406067+00:00"},{"alias_kind":"pith_short_12","alias_value":"HBGA4CS3G57W","created_at":"2026-07-05T11:38:56.406067+00:00"},{"alias_kind":"pith_short_16","alias_value":"HBGA4CS3G57W7IGX","created_at":"2026-07-05T11:38:56.406067+00:00"},{"alias_kind":"pith_short_8","alias_value":"HBGA4CS3","created_at":"2026-07-05T11:38:56.406067+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4","json":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4.json","graph_json":"https://pith.science/api/pith-number/HBGA4CS3G57W7IGXNEIDKUJKO4/graph.json","events_json":"https://pith.science/api/pith-number/HBGA4CS3G57W7IGXNEIDKUJKO4/events.json","paper":"https://pith.science/paper/HBGA4CS3"},"agent_actions":{"view_html":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4","download_json":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4.json","view_paper":"https://pith.science/paper/HBGA4CS3","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=2507.13211&json=true","fetch_graph":"https://pith.science/api/pith-number/HBGA4CS3G57W7IGXNEIDKUJKO4/graph.json","fetch_events":"https://pith.science/api/pith-number/HBGA4CS3G57W7IGXNEIDKUJKO4/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4/action/timestamp_anchor","attest_storage":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4/action/storage_attestation","attest_author":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4/action/author_attestation","sign_citation":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4/action/citation_signature","submit_replication":"https://pith.science/pith/HBGA4CS3G57W7IGXNEIDKUJKO4/action/replication_record"}},"created_at":"2026-07-05T11:38:56.406067+00:00","updated_at":"2026-07-05T11:38:56.406067+00:00"}