{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2015:HDWY6XZ7HEWBF3VEIOOSRAZ2I7","short_pith_number":"pith:HDWY6XZ7","canonical_record":{"source":{"id":"1505.01032","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2015-05-05T15:09:33Z","cross_cats_sorted":["physics.ins-det","q-bio.QM"],"title_canon_sha256":"fda61d4868cb7fbd68e1ece89bf91a437396c0482da41815f60dc0f295f43129","abstract_canon_sha256":"909481ef6945dc3bfebdfba4f9bdf1369d0f7938b05d57920169e67ad020b538"},"schema_version":"1.0"},"canonical_sha256":"38ed8f5f3f392c12eea4439d28833a47efeb8ff2ee8dbbb9a909f0af306e00c2","source":{"kind":"arxiv","id":"1505.01032","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1505.01032","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"arxiv_version","alias_value":"1505.01032v1","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1505.01032","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"pith_short_12","alias_value":"HDWY6XZ7HEWB","created_at":"2026-05-18T12:29:25Z"},{"alias_kind":"pith_short_16","alias_value":"HDWY6XZ7HEWBF3VE","created_at":"2026-05-18T12:29:25Z"},{"alias_kind":"pith_short_8","alias_value":"HDWY6XZ7","created_at":"2026-05-18T12:29:25Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2015:HDWY6XZ7HEWBF3VEIOOSRAZ2I7","target":"record","payload":{"canonical_record":{"source":{"id":"1505.01032","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2015-05-05T15:09:33Z","cross_cats_sorted":["physics.ins-det","q-bio.QM"],"title_canon_sha256":"fda61d4868cb7fbd68e1ece89bf91a437396c0482da41815f60dc0f295f43129","abstract_canon_sha256":"909481ef6945dc3bfebdfba4f9bdf1369d0f7938b05d57920169e67ad020b538"},"schema_version":"1.0"},"canonical_sha256":"38ed8f5f3f392c12eea4439d28833a47efeb8ff2ee8dbbb9a909f0af306e00c2","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T02:16:52.129810Z","signature_b64":"gDezCnjtfReNBn11jcwdPHQyOxl4feMwjL+d76K/rqgJM4HINW3XgvMIpIhhcQeXAazcIgKz7H3NF1C447vMDw==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"38ed8f5f3f392c12eea4439d28833a47efeb8ff2ee8dbbb9a909f0af306e00c2","last_reissued_at":"2026-05-18T02:16:52.128970Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T02:16:52.128970Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1505.01032","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:16:52Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"HQkgBjwfA5O39biBBPnJrHvCd1h/hlX29oX2VV0ART2gtdr4kEm2xK+9S9+q8ZYWbCmS1RI7NeGKifBf5A+GCQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-12T04:57:31.450039Z"},"content_sha256":"8ba3a2ba04d357c387e2100e79086ab4c32ad252eeb018ec1b3f67aed4b9e442","schema_version":"1.0","event_id":"sha256:8ba3a2ba04d357c387e2100e79086ab4c32ad252eeb018ec1b3f67aed4b9e442"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2015:HDWY6XZ7HEWBF3VEIOOSRAZ2I7","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Limitations of Label-Free Sensors in Serum Based Molecular Diagnostics","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.ins-det","q-bio.QM"],"primary_cat":"physics.bio-ph","authors_text":"Manoj M. Varma","submitted_at":"2015-05-05T15:09:33Z","abstract_excerpt":"Immunoassay formats applicable for clinical or point-of-care diagnostics fall into two broad classes. One which uses labeled secondary antibodies for signal transduction and the other which does not require the use of any labels. Comparison of the limits of detection (LoD) reported by these two sensing approaches over a wide range of detection techniques and target molecules in serum revealed that labeled techniques achieve 2-3 orders of magnitude better LoDs. Further, a vast majority of commercial tests and recent examples of technology translations are based on labeled assay formats. In ligh"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1505.01032","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T02:16:52Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"g/G5DWjNnFn9/2UUPFRDzQ9+qiiwdLw2cQ8b7xpoKltz4QNG8xg+i0m/GcaTG+0/hzE+A0G9C3fp5eCeQZ1kBg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-12T04:57:31.450679Z"},"content_sha256":"71b64e868d134e47fd473948c28c08b24474ef9613b9087b3feab633e74c6c30","schema_version":"1.0","event_id":"sha256:71b64e868d134e47fd473948c28c08b24474ef9613b9087b3feab633e74c6c30"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/bundle.json","state_url":"https://pith.science/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-12T04:57:31Z","links":{"resolver":"https://pith.science/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7","bundle":"https://pith.science/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/bundle.json","state":"https://pith.science/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/state.json","well_known_bundle":"https://pith.science/.well-known/pith/HDWY6XZ7HEWBF3VEIOOSRAZ2I7/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2015:HDWY6XZ7HEWBF3VEIOOSRAZ2I7","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"909481ef6945dc3bfebdfba4f9bdf1369d0f7938b05d57920169e67ad020b538","cross_cats_sorted":["physics.ins-det","q-bio.QM"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2015-05-05T15:09:33Z","title_canon_sha256":"fda61d4868cb7fbd68e1ece89bf91a437396c0482da41815f60dc0f295f43129"},"schema_version":"1.0","source":{"id":"1505.01032","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1505.01032","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"arxiv_version","alias_value":"1505.01032v1","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1505.01032","created_at":"2026-05-18T02:16:52Z"},{"alias_kind":"pith_short_12","alias_value":"HDWY6XZ7HEWB","created_at":"2026-05-18T12:29:25Z"},{"alias_kind":"pith_short_16","alias_value":"HDWY6XZ7HEWBF3VE","created_at":"2026-05-18T12:29:25Z"},{"alias_kind":"pith_short_8","alias_value":"HDWY6XZ7","created_at":"2026-05-18T12:29:25Z"}],"graph_snapshots":[{"event_id":"sha256:71b64e868d134e47fd473948c28c08b24474ef9613b9087b3feab633e74c6c30","target":"graph","created_at":"2026-05-18T02:16:52Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Immunoassay formats applicable for clinical or point-of-care diagnostics fall into two broad classes. One which uses labeled secondary antibodies for signal transduction and the other which does not require the use of any labels. Comparison of the limits of detection (LoD) reported by these two sensing approaches over a wide range of detection techniques and target molecules in serum revealed that labeled techniques achieve 2-3 orders of magnitude better LoDs. Further, a vast majority of commercial tests and recent examples of technology translations are based on labeled assay formats. In ligh","authors_text":"Manoj M. Varma","cross_cats":["physics.ins-det","q-bio.QM"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2015-05-05T15:09:33Z","title":"Limitations of Label-Free Sensors in Serum Based Molecular Diagnostics"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1505.01032","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:8ba3a2ba04d357c387e2100e79086ab4c32ad252eeb018ec1b3f67aed4b9e442","target":"record","created_at":"2026-05-18T02:16:52Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"909481ef6945dc3bfebdfba4f9bdf1369d0f7938b05d57920169e67ad020b538","cross_cats_sorted":["physics.ins-det","q-bio.QM"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2015-05-05T15:09:33Z","title_canon_sha256":"fda61d4868cb7fbd68e1ece89bf91a437396c0482da41815f60dc0f295f43129"},"schema_version":"1.0","source":{"id":"1505.01032","kind":"arxiv","version":1}},"canonical_sha256":"38ed8f5f3f392c12eea4439d28833a47efeb8ff2ee8dbbb9a909f0af306e00c2","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"38ed8f5f3f392c12eea4439d28833a47efeb8ff2ee8dbbb9a909f0af306e00c2","first_computed_at":"2026-05-18T02:16:52.128970Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T02:16:52.128970Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"gDezCnjtfReNBn11jcwdPHQyOxl4feMwjL+d76K/rqgJM4HINW3XgvMIpIhhcQeXAazcIgKz7H3NF1C447vMDw==","signature_status":"signed_v1","signed_at":"2026-05-18T02:16:52.129810Z","signed_message":"canonical_sha256_bytes"},"source_id":"1505.01032","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:8ba3a2ba04d357c387e2100e79086ab4c32ad252eeb018ec1b3f67aed4b9e442","sha256:71b64e868d134e47fd473948c28c08b24474ef9613b9087b3feab633e74c6c30"],"state_sha256":"4d7dd4bb80a027242ff2ad9fd89d42db0329545a2b1a3e65ba96f5f9d773e511"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"BHKnDLUKrORxnka8mTypLtpT2vJYPUc7AIRXZRH/1pfuBKECpGyEE6y02hHEvYNr40nEQ4PXoeMbLW2AeWnDCQ==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-12T04:57:31.453934Z","bundle_sha256":"f5938cb9d18aab627ed952bf3aeeb725892c3c79fb68fb793e6fe575de69ad09"}}