{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2025:HHRXFU4RRIAGWYFDGOFZKMP2UE","short_pith_number":"pith:HHRXFU4R","canonical_record":{"source":{"id":"2506.05916","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2025-06-06T09:37:41Z","cross_cats_sorted":["physics.bio-ph","q-bio.MN"],"title_canon_sha256":"88ab2a18490d5cb3d9bdf26b5eab1356a0bc58f7cfa60b3db566b5c546f95b2d","abstract_canon_sha256":"40edff8d8beea03facb802e7b466d7e91b08fc3cac6d727ea47635381e556051"},"schema_version":"1.0"},"canonical_sha256":"39e372d3918a006b60a3338b9531faa12e2711f90e2d871a3d1c7641dc612285","source":{"kind":"arxiv","id":"2506.05916","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2506.05916","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"arxiv_version","alias_value":"2506.05916v1","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2506.05916","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_12","alias_value":"HHRXFU4RRIAG","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_16","alias_value":"HHRXFU4RRIAGWYFD","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_8","alias_value":"HHRXFU4R","created_at":"2026-07-05T11:17:12Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2025:HHRXFU4RRIAGWYFDGOFZKMP2UE","target":"record","payload":{"canonical_record":{"source":{"id":"2506.05916","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2025-06-06T09:37:41Z","cross_cats_sorted":["physics.bio-ph","q-bio.MN"],"title_canon_sha256":"88ab2a18490d5cb3d9bdf26b5eab1356a0bc58f7cfa60b3db566b5c546f95b2d","abstract_canon_sha256":"40edff8d8beea03facb802e7b466d7e91b08fc3cac6d727ea47635381e556051"},"schema_version":"1.0"},"canonical_sha256":"39e372d3918a006b60a3338b9531faa12e2711f90e2d871a3d1c7641dc612285","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T11:17:12.663891Z","signature_b64":"P+LuRIUXP51Y8HZqBkFZSlPsjyxHl3x8fHSZABm5xK7ReiZl8ydtOs5dWEGeoRCERTUFsEAEwEQwXnKwrIF6Bw==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"39e372d3918a006b60a3338b9531faa12e2711f90e2d871a3d1c7641dc612285","last_reissued_at":"2026-07-05T11:17:12.663264Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T11:17:12.663264Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"2506.05916","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T11:17:12Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"aQrKM1S2FaAH+X+nUg4pCAhJndsPk0fdu9MpVaQ0koMjwk7iUMShs6BqMjLQXmSQ9zxAs0aEP9ViGD7m4ssGCg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-13T13:38:12.608086Z"},"content_sha256":"a05ceb82bb9c12d6e22bb64df64ed76d710df8855ead3e2939f13cf40d736b84","schema_version":"1.0","event_id":"sha256:a05ceb82bb9c12d6e22bb64df64ed76d710df8855ead3e2939f13cf40d736b84"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2025:HHRXFU4RRIAGWYFDGOFZKMP2UE","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Single-cell metabolic flux analysis reveals coexisting optimal sub-groups, cross-feeding, and mixotrophy in a cyanobacterial population","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.bio-ph","q-bio.MN"],"primary_cat":"q-bio.PE","authors_text":"Andrea De Martino, Ari\\'an Ferrero-Fern\\'andez, Daniele De Martino, David A. Fike, Harrison B. Smith, Lucia Gastoldi, Paula Prondzinsky, Shawn Erin McGlynn","submitted_at":"2025-06-06T09:37:41Z","abstract_excerpt":"We derive a single-cell level understanding of metabolism in an isogenic cyanobacterial population by integrating secondary ion mass spectrometry (SIMS) derived multi-isotope uptake measurements of Synechocystis sp. PCC6803 with a statistical inference protocol based on Liebig's law of the minimum, the maximum entropy principle, and constraint-based modeling. We find the population is structured in two metabolically distinct clusters: cells optimizing carbon yield while excessively turning over nitrogen, and cells which act reciprocally, optimizing nitrogen yield and excessively turning over c"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2506.05916","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2506.05916/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T11:17:12Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"EpBrfopSdyCCdoynaZBKFyHJ8zkkqvn2c+A06pdBgxVxsBl5PlygBJxbAaszVvYwaos9n/eisB0V7ICn/Zy0Aw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-07-13T13:38:12.608477Z"},"content_sha256":"d52679936ac19820ac71424bc00110325c69ea91621b8de875df0d6d2c71aac0","schema_version":"1.0","event_id":"sha256:d52679936ac19820ac71424bc00110325c69ea91621b8de875df0d6d2c71aac0"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/bundle.json","state_url":"https://pith.science/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-07-13T13:38:12Z","links":{"resolver":"https://pith.science/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE","bundle":"https://pith.science/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/bundle.json","state":"https://pith.science/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/state.json","well_known_bundle":"https://pith.science/.well-known/pith/HHRXFU4RRIAGWYFDGOFZKMP2UE/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2025:HHRXFU4RRIAGWYFDGOFZKMP2UE","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"40edff8d8beea03facb802e7b466d7e91b08fc3cac6d727ea47635381e556051","cross_cats_sorted":["physics.bio-ph","q-bio.MN"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2025-06-06T09:37:41Z","title_canon_sha256":"88ab2a18490d5cb3d9bdf26b5eab1356a0bc58f7cfa60b3db566b5c546f95b2d"},"schema_version":"1.0","source":{"id":"2506.05916","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2506.05916","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"arxiv_version","alias_value":"2506.05916v1","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2506.05916","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_12","alias_value":"HHRXFU4RRIAG","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_16","alias_value":"HHRXFU4RRIAGWYFD","created_at":"2026-07-05T11:17:12Z"},{"alias_kind":"pith_short_8","alias_value":"HHRXFU4R","created_at":"2026-07-05T11:17:12Z"}],"graph_snapshots":[{"event_id":"sha256:d52679936ac19820ac71424bc00110325c69ea91621b8de875df0d6d2c71aac0","target":"graph","created_at":"2026-07-05T11:17:12Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"integrity":{"available":true,"clean":true,"detectors_run":[],"endpoint":"/pith/2506.05916/integrity.json","findings":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938","summary":{"advisory":0,"by_detector":{},"critical":0,"informational":0}},"paper":{"abstract_excerpt":"We derive a single-cell level understanding of metabolism in an isogenic cyanobacterial population by integrating secondary ion mass spectrometry (SIMS) derived multi-isotope uptake measurements of Synechocystis sp. PCC6803 with a statistical inference protocol based on Liebig's law of the minimum, the maximum entropy principle, and constraint-based modeling. We find the population is structured in two metabolically distinct clusters: cells optimizing carbon yield while excessively turning over nitrogen, and cells which act reciprocally, optimizing nitrogen yield and excessively turning over c","authors_text":"Andrea De Martino, Ari\\'an Ferrero-Fern\\'andez, Daniele De Martino, David A. Fike, Harrison B. Smith, Lucia Gastoldi, Paula Prondzinsky, Shawn Erin McGlynn","cross_cats":["physics.bio-ph","q-bio.MN"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2025-06-06T09:37:41Z","title":"Single-cell metabolic flux analysis reveals coexisting optimal sub-groups, cross-feeding, and mixotrophy in a cyanobacterial population"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2506.05916","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:a05ceb82bb9c12d6e22bb64df64ed76d710df8855ead3e2939f13cf40d736b84","target":"record","created_at":"2026-07-05T11:17:12Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"40edff8d8beea03facb802e7b466d7e91b08fc3cac6d727ea47635381e556051","cross_cats_sorted":["physics.bio-ph","q-bio.MN"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.PE","submitted_at":"2025-06-06T09:37:41Z","title_canon_sha256":"88ab2a18490d5cb3d9bdf26b5eab1356a0bc58f7cfa60b3db566b5c546f95b2d"},"schema_version":"1.0","source":{"id":"2506.05916","kind":"arxiv","version":1}},"canonical_sha256":"39e372d3918a006b60a3338b9531faa12e2711f90e2d871a3d1c7641dc612285","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"39e372d3918a006b60a3338b9531faa12e2711f90e2d871a3d1c7641dc612285","first_computed_at":"2026-07-05T11:17:12.663264Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-07-05T11:17:12.663264Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"P+LuRIUXP51Y8HZqBkFZSlPsjyxHl3x8fHSZABm5xK7ReiZl8ydtOs5dWEGeoRCERTUFsEAEwEQwXnKwrIF6Bw==","signature_status":"signed_v1","signed_at":"2026-07-05T11:17:12.663891Z","signed_message":"canonical_sha256_bytes"},"source_id":"2506.05916","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:a05ceb82bb9c12d6e22bb64df64ed76d710df8855ead3e2939f13cf40d736b84","sha256:d52679936ac19820ac71424bc00110325c69ea91621b8de875df0d6d2c71aac0"],"state_sha256":"5b2b576eca072e9751b34f92b7aa0b02a53044016bd9ea4235ed361bc7c1835d"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"xI9ygA3UKLJpuO37kl0NZ+sVk93U6fwRoOA2sPsbBkkUMdUXiYQ4HU/VT9kvcg9f7zP7tjuWp6PZavhB8vOEAA==","signed_message":"bundle_sha256_bytes","signed_at":"2026-07-13T13:38:12.610598Z","bundle_sha256":"14d32aaee73bf961febcf31f17212f683c871ce6d3ff579bc0e34e0ed15c061d"}}