{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2006:IRRQZ3W6C42EFTTMTII5AK2ISY","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"6ecd3867723efcba1c97051e7fb2f323e8149b95709e53b5efd7dc62e128fb42","cross_cats_sorted":[],"license":"","primary_cat":"q-bio.GN","submitted_at":"2006-11-24T12:51:38Z","title_canon_sha256":"ef545ef018a6bcae158deb1df1973ad79990df23c318fcb6b93aff686781706b"},"schema_version":"1.0","source":{"id":"q-bio/0611079","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"q-bio/0611079","created_at":"2026-05-18T01:08:45Z"},{"alias_kind":"arxiv_version","alias_value":"q-bio/0611079v1","created_at":"2026-05-18T01:08:45Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.q-bio/0611079","created_at":"2026-05-18T01:08:45Z"},{"alias_kind":"pith_short_12","alias_value":"IRRQZ3W6C42E","created_at":"2026-05-18T12:25:54Z"},{"alias_kind":"pith_short_16","alias_value":"IRRQZ3W6C42EFTTM","created_at":"2026-05-18T12:25:54Z"},{"alias_kind":"pith_short_8","alias_value":"IRRQZ3W6","created_at":"2026-05-18T12:25:54Z"}],"graph_snapshots":[{"event_id":"sha256:05ef27780f941b1da5083993f7bb00d83cc742be6725945f160cb58f3f75bd28","target":"graph","created_at":"2026-05-18T01:08:45Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis was investigated. Cysteine blocking greatly increased resolution and decreased streaking, especially in the basic region of the gels. Two strategies for cysteine blocking were found to be efficient: classical alkylation with maleimide derivatives and mixed disulfide exchange with an excess of a low molecular weight disulfide. The effect on resolution was significant enough to allow correct resolution of basic proteins with in-gel rehydration on wide gradients (e.g. 3-10 and 4-12), but anodic ","authors_text":"Alain van Dorsselaer, Chistophe Tastet, Emmanuelle Leize-Wagner, H\\'el\\`ene Diemer, Mireille Chevallet, Sylvie Luche, Thierry Rabilloud","cross_cats":[],"headline":"","license":"","primary_cat":"q-bio.GN","submitted_at":"2006-11-24T12:51:38Z","title":"About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"q-bio/0611079","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:46ae425f9467ad09bd01e1b8d2d8cdf49ba08d09d3a590d7cb2657fea84bdda1","target":"record","created_at":"2026-05-18T01:08:45Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"6ecd3867723efcba1c97051e7fb2f323e8149b95709e53b5efd7dc62e128fb42","cross_cats_sorted":[],"license":"","primary_cat":"q-bio.GN","submitted_at":"2006-11-24T12:51:38Z","title_canon_sha256":"ef545ef018a6bcae158deb1df1973ad79990df23c318fcb6b93aff686781706b"},"schema_version":"1.0","source":{"id":"q-bio/0611079","kind":"arxiv","version":1}},"canonical_sha256":"44630ceede173442ce6c9a11d02b489606d46d6d824a1e1f85610c62c676903e","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"44630ceede173442ce6c9a11d02b489606d46d6d824a1e1f85610c62c676903e","first_computed_at":"2026-05-18T01:08:45.955204Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T01:08:45.955204Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"JJxec4a2QzKYM1fGqIdBxAxsE+/7GVxw3Tvge8Hjm33Xify4pxhwBE8AIGj9wRjQ515EMFgmOkUNEWHKx2TgBg==","signature_status":"signed_v1","signed_at":"2026-05-18T01:08:45.955643Z","signed_message":"canonical_sha256_bytes"},"source_id":"q-bio/0611079","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:46ae425f9467ad09bd01e1b8d2d8cdf49ba08d09d3a590d7cb2657fea84bdda1","sha256:05ef27780f941b1da5083993f7bb00d83cc742be6725945f160cb58f3f75bd28"],"state_sha256":"f6c4c4e2ecb531dff479dbd3dbc5be396d9c7f10fd2d0dbd7b6b5898b0bfd591"}