{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2016:JMBHF3N7ES7UN6TMQMSB26FVQR","short_pith_number":"pith:JMBHF3N7","schema_version":"1.0","canonical_sha256":"4b0272edbf24bf46fa6c83241d78b5845550ffa60bb075ad890e3ccfb3a7fa5f","source":{"kind":"arxiv","id":"1606.00620","version":1},"attestation_state":"computed","paper":{"title":"What is the 'minimum inhibitory concentration' (MIC) of pexiganan acting on Escherichia coli? - A cautionary case study","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"Alys K Jepson, Jana Schwarz-Linek, Lloyd Ryan, Maxim G Ryadnov, Wilson C K Poon","submitted_at":"2016-06-02T11:03:04Z","abstract_excerpt":"We measured the minimum inhibitory concentration (MIC) of the antimicrobial peptide pexiganan acting on Escherichia coli, and report an intrinsic variability in such measurements. These results led to a detailed study of the effect of pexiganan on the growth curve of E. coli, using a plate reader and manual plating (i.e. time-kill curves). The measured growth curves, together with single-cell observations and peptide depletion assays, suggested that addition of a sub-MIC concentration of pexiganan to a population of this bacterium killed a fraction of the cells, reducing peptide activity durin"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1606.00620","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2016-06-02T11:03:04Z","cross_cats_sorted":[],"title_canon_sha256":"0dd52899bfe9f6acca3a676840cdad287a3e66059268728ff66d98324a89c063","abstract_canon_sha256":"462bf053c0c0bec7d8d74ab87219fcb3df28c41ee98ab941d5c7dee49d81699d"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T01:13:04.053603Z","signature_b64":"QK4PGeKFmnja8bbsFFQkCmlNxvWsJsKJMrKGMyBvZcQ2weSgJ3s3D172ga6Ig0juqZn44Do60WgR9YMcq8UoCg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"4b0272edbf24bf46fa6c83241d78b5845550ffa60bb075ad890e3ccfb3a7fa5f","last_reissued_at":"2026-05-18T01:13:04.052955Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T01:13:04.052955Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"What is the 'minimum inhibitory concentration' (MIC) of pexiganan acting on Escherichia coli? - A cautionary case study","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"physics.bio-ph","authors_text":"Alys K Jepson, Jana Schwarz-Linek, Lloyd Ryan, Maxim G Ryadnov, Wilson C K Poon","submitted_at":"2016-06-02T11:03:04Z","abstract_excerpt":"We measured the minimum inhibitory concentration (MIC) of the antimicrobial peptide pexiganan acting on Escherichia coli, and report an intrinsic variability in such measurements. These results led to a detailed study of the effect of pexiganan on the growth curve of E. coli, using a plate reader and manual plating (i.e. time-kill curves). The measured growth curves, together with single-cell observations and peptide depletion assays, suggested that addition of a sub-MIC concentration of pexiganan to a population of this bacterium killed a fraction of the cells, reducing peptide activity durin"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1606.00620","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1606.00620","created_at":"2026-05-18T01:13:04.053053+00:00"},{"alias_kind":"arxiv_version","alias_value":"1606.00620v1","created_at":"2026-05-18T01:13:04.053053+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1606.00620","created_at":"2026-05-18T01:13:04.053053+00:00"},{"alias_kind":"pith_short_12","alias_value":"JMBHF3N7ES7U","created_at":"2026-05-18T12:30:25.849896+00:00"},{"alias_kind":"pith_short_16","alias_value":"JMBHF3N7ES7UN6TM","created_at":"2026-05-18T12:30:25.849896+00:00"},{"alias_kind":"pith_short_8","alias_value":"JMBHF3N7","created_at":"2026-05-18T12:30:25.849896+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR","json":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR.json","graph_json":"https://pith.science/api/pith-number/JMBHF3N7ES7UN6TMQMSB26FVQR/graph.json","events_json":"https://pith.science/api/pith-number/JMBHF3N7ES7UN6TMQMSB26FVQR/events.json","paper":"https://pith.science/paper/JMBHF3N7"},"agent_actions":{"view_html":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR","download_json":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR.json","view_paper":"https://pith.science/paper/JMBHF3N7","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1606.00620&json=true","fetch_graph":"https://pith.science/api/pith-number/JMBHF3N7ES7UN6TMQMSB26FVQR/graph.json","fetch_events":"https://pith.science/api/pith-number/JMBHF3N7ES7UN6TMQMSB26FVQR/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR/action/timestamp_anchor","attest_storage":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR/action/storage_attestation","attest_author":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR/action/author_attestation","sign_citation":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR/action/citation_signature","submit_replication":"https://pith.science/pith/JMBHF3N7ES7UN6TMQMSB26FVQR/action/replication_record"}},"created_at":"2026-05-18T01:13:04.053053+00:00","updated_at":"2026-05-18T01:13:04.053053+00:00"}