{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2012:JRORHP2VKKBHRFF47SH44UNS4J","short_pith_number":"pith:JRORHP2V","canonical_record":{"source":{"id":"1206.3003","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-06-14T04:57:18Z","cross_cats_sorted":[],"title_canon_sha256":"129223d167966c3de4d8d91d0abc9125aa5b85195f3981d810aa5d5af1b3a2b7","abstract_canon_sha256":"ebe35828cfe38cdeb9d62f4868a10d4d48fd9270761af33536288a7f70aded36"},"schema_version":"1.0"},"canonical_sha256":"4c5d13bf5552827894bcfc8fce51b2e2660e09c95652d3274a65737557a9f394","source":{"kind":"arxiv","id":"1206.3003","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1206.3003","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"arxiv_version","alias_value":"1206.3003v1","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1206.3003","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"pith_short_12","alias_value":"JRORHP2VKKBH","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_16","alias_value":"JRORHP2VKKBHRFF4","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_8","alias_value":"JRORHP2V","created_at":"2026-05-18T12:27:11Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2012:JRORHP2VKKBHRFF47SH44UNS4J","target":"record","payload":{"canonical_record":{"source":{"id":"1206.3003","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-06-14T04:57:18Z","cross_cats_sorted":[],"title_canon_sha256":"129223d167966c3de4d8d91d0abc9125aa5b85195f3981d810aa5d5af1b3a2b7","abstract_canon_sha256":"ebe35828cfe38cdeb9d62f4868a10d4d48fd9270761af33536288a7f70aded36"},"schema_version":"1.0"},"canonical_sha256":"4c5d13bf5552827894bcfc8fce51b2e2660e09c95652d3274a65737557a9f394","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:53:33.122705Z","signature_b64":"a4GX+WPkzUUBVrmtmKv1OY1ClkZSx4d1GLq5qhsTO6CqQ5Xs9PHitCyzXl9VcNP0qp4tt9E04eShquJSQKuKBQ==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"4c5d13bf5552827894bcfc8fce51b2e2660e09c95652d3274a65737557a9f394","last_reissued_at":"2026-05-18T03:53:33.121515Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:53:33.121515Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1206.3003","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:53:33Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"Ndkzeb/InQDNlVKoMhlhxG+9GExFKvHNAcQc3NdV2w6t6RODLEvsWgmFEzXN1EhwOiQyjD9iEoYTZKg4VprJBQ==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-28T13:22:02.985723Z"},"content_sha256":"1122f45dbee0c1d5c1173c2e99332152a196b7c4af58920a68b1a34253869156","schema_version":"1.0","event_id":"sha256:1122f45dbee0c1d5c1173c2e99332152a196b7c4af58920a68b1a34253869156"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2012:JRORHP2VKKBHRFF47SH44UNS4J","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"The Whereabouts of 2D Gels in Quantitative Proteomics","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.GN","authors_text":"Thierry Rabilloud (LCBM)","submitted_at":"2012-06-14T04:57:18Z","abstract_excerpt":"Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using 2D gels is critically dependent on the performances of the protein detection methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods (radioactivity, dyes, fluorescen"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1206.3003","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:53:33Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"MNW08ZOglQrYRaaZrckh57kPcLikWd1V/o5gOSk+X/Jz/5lhI/jbk8nMtLYHUC22+/7FXsqEE3/t2cIgSbk/BA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-28T13:22:02.986113Z"},"content_sha256":"05992da69772e644887258e26a9c27cae3485aed884aaa405ee56ead08db352a","schema_version":"1.0","event_id":"sha256:05992da69772e644887258e26a9c27cae3485aed884aaa405ee56ead08db352a"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/JRORHP2VKKBHRFF47SH44UNS4J/bundle.json","state_url":"https://pith.science/pith/JRORHP2VKKBHRFF47SH44UNS4J/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/JRORHP2VKKBHRFF47SH44UNS4J/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-28T13:22:02Z","links":{"resolver":"https://pith.science/pith/JRORHP2VKKBHRFF47SH44UNS4J","bundle":"https://pith.science/pith/JRORHP2VKKBHRFF47SH44UNS4J/bundle.json","state":"https://pith.science/pith/JRORHP2VKKBHRFF47SH44UNS4J/state.json","well_known_bundle":"https://pith.science/.well-known/pith/JRORHP2VKKBHRFF47SH44UNS4J/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2012:JRORHP2VKKBHRFF47SH44UNS4J","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"ebe35828cfe38cdeb9d62f4868a10d4d48fd9270761af33536288a7f70aded36","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-06-14T04:57:18Z","title_canon_sha256":"129223d167966c3de4d8d91d0abc9125aa5b85195f3981d810aa5d5af1b3a2b7"},"schema_version":"1.0","source":{"id":"1206.3003","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1206.3003","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"arxiv_version","alias_value":"1206.3003v1","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1206.3003","created_at":"2026-05-18T03:53:33Z"},{"alias_kind":"pith_short_12","alias_value":"JRORHP2VKKBH","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_16","alias_value":"JRORHP2VKKBHRFF4","created_at":"2026-05-18T12:27:11Z"},{"alias_kind":"pith_short_8","alias_value":"JRORHP2V","created_at":"2026-05-18T12:27:11Z"}],"graph_snapshots":[{"event_id":"sha256:05992da69772e644887258e26a9c27cae3485aed884aaa405ee56ead08db352a","target":"graph","created_at":"2026-05-18T03:53:33Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using 2D gels is critically dependent on the performances of the protein detection methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods (radioactivity, dyes, fluorescen","authors_text":"Thierry Rabilloud (LCBM)","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-06-14T04:57:18Z","title":"The Whereabouts of 2D Gels in Quantitative Proteomics"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1206.3003","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:1122f45dbee0c1d5c1173c2e99332152a196b7c4af58920a68b1a34253869156","target":"record","created_at":"2026-05-18T03:53:33Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"ebe35828cfe38cdeb9d62f4868a10d4d48fd9270761af33536288a7f70aded36","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-06-14T04:57:18Z","title_canon_sha256":"129223d167966c3de4d8d91d0abc9125aa5b85195f3981d810aa5d5af1b3a2b7"},"schema_version":"1.0","source":{"id":"1206.3003","kind":"arxiv","version":1}},"canonical_sha256":"4c5d13bf5552827894bcfc8fce51b2e2660e09c95652d3274a65737557a9f394","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"4c5d13bf5552827894bcfc8fce51b2e2660e09c95652d3274a65737557a9f394","first_computed_at":"2026-05-18T03:53:33.121515Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T03:53:33.121515Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"a4GX+WPkzUUBVrmtmKv1OY1ClkZSx4d1GLq5qhsTO6CqQ5Xs9PHitCyzXl9VcNP0qp4tt9E04eShquJSQKuKBQ==","signature_status":"signed_v1","signed_at":"2026-05-18T03:53:33.122705Z","signed_message":"canonical_sha256_bytes"},"source_id":"1206.3003","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:1122f45dbee0c1d5c1173c2e99332152a196b7c4af58920a68b1a34253869156","sha256:05992da69772e644887258e26a9c27cae3485aed884aaa405ee56ead08db352a"],"state_sha256":"c3790ea7723d2181df7d20a81855439156c1b020152857e551c7a8a99011bcd4"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"uS+6WJFZxWfq6xWdmiRUqXq5YEcsBGSxncYA+J94oVTAqUekf6u80OR/ajgYRF9a/fjWGoX+LPDpIdElSqINAw==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-28T13:22:02.987967Z","bundle_sha256":"6d20336f0fb4e6799d5a14ffe1a3274d1cb9e421b1945806c5ade3b89ea2eb7e"}}