{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2013:QDLH4LIS66C3UO4PDRBCMTVZUM","short_pith_number":"pith:QDLH4LIS","canonical_record":{"source":{"id":"1307.2737","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2013-07-10T10:05:30Z","cross_cats_sorted":["cond-mat.soft","physics.bio-ph"],"title_canon_sha256":"b5dc0c5896c8c2316ffaa94cbb760df03f73d27ef1ca9557e0a3d78861b2dab4","abstract_canon_sha256":"508bee4579d4fa5255abcfe77251bdea3e942df99debee1587052ed4e8fe7939"},"schema_version":"1.0"},"canonical_sha256":"80d67e2d12f785ba3b8f1c42264eb9a32fcd2f15e59cd09606de10a919870827","source":{"kind":"arxiv","id":"1307.2737","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1307.2737","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"arxiv_version","alias_value":"1307.2737v1","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1307.2737","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"pith_short_12","alias_value":"QDLH4LIS66C3","created_at":"2026-05-18T12:27:57Z"},{"alias_kind":"pith_short_16","alias_value":"QDLH4LIS66C3UO4P","created_at":"2026-05-18T12:27:57Z"},{"alias_kind":"pith_short_8","alias_value":"QDLH4LIS","created_at":"2026-05-18T12:27:57Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2013:QDLH4LIS66C3UO4PDRBCMTVZUM","target":"record","payload":{"canonical_record":{"source":{"id":"1307.2737","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2013-07-10T10:05:30Z","cross_cats_sorted":["cond-mat.soft","physics.bio-ph"],"title_canon_sha256":"b5dc0c5896c8c2316ffaa94cbb760df03f73d27ef1ca9557e0a3d78861b2dab4","abstract_canon_sha256":"508bee4579d4fa5255abcfe77251bdea3e942df99debee1587052ed4e8fe7939"},"schema_version":"1.0"},"canonical_sha256":"80d67e2d12f785ba3b8f1c42264eb9a32fcd2f15e59cd09606de10a919870827","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:18:50.561139Z","signature_b64":"mzFJu9Ipgy2yK/x9UtM5PWgLwHD+XdouCZp9+3XwGg5J7+J+Qdrt98oPmyWBQosd+QPKWVtTeLZbywLojOGIDg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"80d67e2d12f785ba3b8f1c42264eb9a32fcd2f15e59cd09606de10a919870827","last_reissued_at":"2026-05-18T03:18:50.560406Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:18:50.560406Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1307.2737","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:18:50Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"UX/kVw3IZUqEIfCnK6aXPllc4GfEBy+wg0gw69heRS32HrCMYMFRjSHE0GRqw6GzHgCsSN26SJ4s66WA3zDdBw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-23T10:31:39.509697Z"},"content_sha256":"4a5e6952abe535fbdd13c0ff6b78198006069ca5d021a1b4c7adbd804f0ffa01","schema_version":"1.0","event_id":"sha256:4a5e6952abe535fbdd13c0ff6b78198006069ca5d021a1b4c7adbd804f0ffa01"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2013:QDLH4LIS66C3UO4PDRBCMTVZUM","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Solvent Sensitivity of Protein Unfolding: Study of Chicken Villin Headpiece Subdomain in Water-Ethanol and Water-DMSO Mixtures","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["cond-mat.soft","physics.bio-ph"],"primary_cat":"q-bio.BM","authors_text":"Biman Bagchi, Rikhia Ghosh, Susmita Roy","submitted_at":"2013-07-10T10:05:30Z","abstract_excerpt":"In the present work we study and compare unfolding of a small protein, chicken villin headpiece (HP-36) in two different aqueous binary mixtures, namely water-ethanol (EtOH) and water-dimethyl sulphoxide (DMSO). In both the binary mixtures, HP-36 is found to unfold (fully or partially, depending on the mixture) under ambient conditions, that otherwise requires temperature as high as ~600 K to denature in pure aqueous solvent. In all the cases, first step of unfolding is found to be similar, i.e. separation of the cluster formed by three hydrophobic (phenylalanine) residues, namely Phe-7, Phe-1"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1307.2737","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:18:50Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"YDGhqk0g5layAMxhfwjPxFkvmmBcVPIWwaEH7i5rYq9Ame8Q8jESEtaeJr+in5nxXPc0txny24IdZuWsu70UCw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-23T10:31:39.510085Z"},"content_sha256":"4058188df7fae0daf3df8a44ea3026a0c97a8fea58c58aaff8e37b0c4511e359","schema_version":"1.0","event_id":"sha256:4058188df7fae0daf3df8a44ea3026a0c97a8fea58c58aaff8e37b0c4511e359"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/bundle.json","state_url":"https://pith.science/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-23T10:31:39Z","links":{"resolver":"https://pith.science/pith/QDLH4LIS66C3UO4PDRBCMTVZUM","bundle":"https://pith.science/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/bundle.json","state":"https://pith.science/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/state.json","well_known_bundle":"https://pith.science/.well-known/pith/QDLH4LIS66C3UO4PDRBCMTVZUM/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2013:QDLH4LIS66C3UO4PDRBCMTVZUM","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"508bee4579d4fa5255abcfe77251bdea3e942df99debee1587052ed4e8fe7939","cross_cats_sorted":["cond-mat.soft","physics.bio-ph"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2013-07-10T10:05:30Z","title_canon_sha256":"b5dc0c5896c8c2316ffaa94cbb760df03f73d27ef1ca9557e0a3d78861b2dab4"},"schema_version":"1.0","source":{"id":"1307.2737","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1307.2737","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"arxiv_version","alias_value":"1307.2737v1","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1307.2737","created_at":"2026-05-18T03:18:50Z"},{"alias_kind":"pith_short_12","alias_value":"QDLH4LIS66C3","created_at":"2026-05-18T12:27:57Z"},{"alias_kind":"pith_short_16","alias_value":"QDLH4LIS66C3UO4P","created_at":"2026-05-18T12:27:57Z"},{"alias_kind":"pith_short_8","alias_value":"QDLH4LIS","created_at":"2026-05-18T12:27:57Z"}],"graph_snapshots":[{"event_id":"sha256:4058188df7fae0daf3df8a44ea3026a0c97a8fea58c58aaff8e37b0c4511e359","target":"graph","created_at":"2026-05-18T03:18:50Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"In the present work we study and compare unfolding of a small protein, chicken villin headpiece (HP-36) in two different aqueous binary mixtures, namely water-ethanol (EtOH) and water-dimethyl sulphoxide (DMSO). In both the binary mixtures, HP-36 is found to unfold (fully or partially, depending on the mixture) under ambient conditions, that otherwise requires temperature as high as ~600 K to denature in pure aqueous solvent. In all the cases, first step of unfolding is found to be similar, i.e. separation of the cluster formed by three hydrophobic (phenylalanine) residues, namely Phe-7, Phe-1","authors_text":"Biman Bagchi, Rikhia Ghosh, Susmita Roy","cross_cats":["cond-mat.soft","physics.bio-ph"],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2013-07-10T10:05:30Z","title":"Solvent Sensitivity of Protein Unfolding: Study of Chicken Villin Headpiece Subdomain in Water-Ethanol and Water-DMSO Mixtures"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1307.2737","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:4a5e6952abe535fbdd13c0ff6b78198006069ca5d021a1b4c7adbd804f0ffa01","target":"record","created_at":"2026-05-18T03:18:50Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"508bee4579d4fa5255abcfe77251bdea3e942df99debee1587052ed4e8fe7939","cross_cats_sorted":["cond-mat.soft","physics.bio-ph"],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.BM","submitted_at":"2013-07-10T10:05:30Z","title_canon_sha256":"b5dc0c5896c8c2316ffaa94cbb760df03f73d27ef1ca9557e0a3d78861b2dab4"},"schema_version":"1.0","source":{"id":"1307.2737","kind":"arxiv","version":1}},"canonical_sha256":"80d67e2d12f785ba3b8f1c42264eb9a32fcd2f15e59cd09606de10a919870827","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"80d67e2d12f785ba3b8f1c42264eb9a32fcd2f15e59cd09606de10a919870827","first_computed_at":"2026-05-18T03:18:50.560406Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T03:18:50.560406Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"mzFJu9Ipgy2yK/x9UtM5PWgLwHD+XdouCZp9+3XwGg5J7+J+Qdrt98oPmyWBQosd+QPKWVtTeLZbywLojOGIDg==","signature_status":"signed_v1","signed_at":"2026-05-18T03:18:50.561139Z","signed_message":"canonical_sha256_bytes"},"source_id":"1307.2737","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:4a5e6952abe535fbdd13c0ff6b78198006069ca5d021a1b4c7adbd804f0ffa01","sha256:4058188df7fae0daf3df8a44ea3026a0c97a8fea58c58aaff8e37b0c4511e359"],"state_sha256":"3b893a008ebdd409afab9cb738d8110fbe017878d9f84ab3640d647a0bf2b4e5"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"JdT1UZd8K1MQuobJjj6BxhPUycTfwB1DWn28l1BW5JyXiyccSmTcNAnVpePDJtVF3aJspy2n1CUzczNza3l6DA==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-23T10:31:39.512168Z","bundle_sha256":"20dfa23feb9c0985bb4433cafaa813809bb5a5fe540a8e586376bf2263b90639"}}