{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2023:SS5W4ILYSPVO7LN6B6E6C763EG","short_pith_number":"pith:SS5W4ILY","schema_version":"1.0","canonical_sha256":"94bb6e217893eaefadbe0f89e17fdb21bac04d701f722a878922f419bc049b45","source":{"kind":"arxiv","id":"2304.05802","version":1},"attestation_state":"computed","paper":{"title":"Nanocrystallites Modulate Intermolecular Interactions in Cryoprotected Protein Solutions","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":["physics.bio-ph","physics.chem-ph"],"primary_cat":"cond-mat.soft","authors_text":"Christian Gutt, Fivos Perakis, Hailong Li, Katrin Amann-Winkel, Maddalena Bin, Malte Blankenburg, Mariia Filianina, Mario Reiser, Sharon Berkowicz, Sonja Timmermann","submitted_at":"2023-04-12T12:29:01Z","abstract_excerpt":"Studying protein interactions at low temperatures has important implications for optimizing cryostorage processes of biological tissue, food, and protein-based drugs. One of the major challenges is related to the formation of ice nanocrystals which can occur even in the presence of cryoprotectants and can lead to protein denaturation. Here, using a combination of small- and wide-angle X-ray scattering (SAXS and WAXS), we investigate the structural evolution of concentrated Lysozyme solutions in a cryoprotected glycerol-water mixture upon cooling from room temperature (T=300 K) down to cryogeni"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"2304.05802","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by/4.0/","primary_cat":"cond-mat.soft","submitted_at":"2023-04-12T12:29:01Z","cross_cats_sorted":["physics.bio-ph","physics.chem-ph"],"title_canon_sha256":"c32bafbc27595f5979468f79221506f4761fc5664bd210d5129ad6e0a09ba97a","abstract_canon_sha256":"75bd664ba428247b733649aed005b4981c873936bee64787f92e6f78723e37fe"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T06:00:23.750900Z","signature_b64":"W8JsRVNwzS4O6JG+9b8jTdaMybdB7p0TuhvZ4iNNoiIHdjB6ykFvr5AQhl2ik8XvZ5jJY7/PsibvizN6o+HWAg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"94bb6e217893eaefadbe0f89e17fdb21bac04d701f722a878922f419bc049b45","last_reissued_at":"2026-07-05T06:00:23.750552Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T06:00:23.750552Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Nanocrystallites Modulate Intermolecular Interactions in Cryoprotected Protein Solutions","license":"http://creativecommons.org/licenses/by/4.0/","headline":"","cross_cats":["physics.bio-ph","physics.chem-ph"],"primary_cat":"cond-mat.soft","authors_text":"Christian Gutt, Fivos Perakis, Hailong Li, Katrin Amann-Winkel, Maddalena Bin, Malte Blankenburg, Mariia Filianina, Mario Reiser, Sharon Berkowicz, Sonja Timmermann","submitted_at":"2023-04-12T12:29:01Z","abstract_excerpt":"Studying protein interactions at low temperatures has important implications for optimizing cryostorage processes of biological tissue, food, and protein-based drugs. One of the major challenges is related to the formation of ice nanocrystals which can occur even in the presence of cryoprotectants and can lead to protein denaturation. Here, using a combination of small- and wide-angle X-ray scattering (SAXS and WAXS), we investigate the structural evolution of concentrated Lysozyme solutions in a cryoprotected glycerol-water mixture upon cooling from room temperature (T=300 K) down to cryogeni"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2304.05802","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2304.05802/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"2304.05802","created_at":"2026-07-05T06:00:23.750614+00:00"},{"alias_kind":"arxiv_version","alias_value":"2304.05802v1","created_at":"2026-07-05T06:00:23.750614+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2304.05802","created_at":"2026-07-05T06:00:23.750614+00:00"},{"alias_kind":"pith_short_12","alias_value":"SS5W4ILYSPVO","created_at":"2026-07-05T06:00:23.750614+00:00"},{"alias_kind":"pith_short_16","alias_value":"SS5W4ILYSPVO7LN6","created_at":"2026-07-05T06:00:23.750614+00:00"},{"alias_kind":"pith_short_8","alias_value":"SS5W4ILY","created_at":"2026-07-05T06:00:23.750614+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG","json":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG.json","graph_json":"https://pith.science/api/pith-number/SS5W4ILYSPVO7LN6B6E6C763EG/graph.json","events_json":"https://pith.science/api/pith-number/SS5W4ILYSPVO7LN6B6E6C763EG/events.json","paper":"https://pith.science/paper/SS5W4ILY"},"agent_actions":{"view_html":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG","download_json":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG.json","view_paper":"https://pith.science/paper/SS5W4ILY","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=2304.05802&json=true","fetch_graph":"https://pith.science/api/pith-number/SS5W4ILYSPVO7LN6B6E6C763EG/graph.json","fetch_events":"https://pith.science/api/pith-number/SS5W4ILYSPVO7LN6B6E6C763EG/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG/action/timestamp_anchor","attest_storage":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG/action/storage_attestation","attest_author":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG/action/author_attestation","sign_citation":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG/action/citation_signature","submit_replication":"https://pith.science/pith/SS5W4ILYSPVO7LN6B6E6C763EG/action/replication_record"}},"created_at":"2026-07-05T06:00:23.750614+00:00","updated_at":"2026-07-05T06:00:23.750614+00:00"}