{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2017:TSOHQTERUF6OPC7L4WMZD5NUVU","short_pith_number":"pith:TSOHQTER","schema_version":"1.0","canonical_sha256":"9c9c784c91a17ce78bebe59991f5b4ad2c57cabbc4173bfacd20d2c0a528c0f6","source":{"kind":"arxiv","id":"1703.00895","version":2},"attestation_state":"computed","paper":{"title":"Absolute bacterial cell enumeration using flow cytometry","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.bio-ph"],"primary_cat":"q-bio.QM","authors_text":"Cushla McGoverin, Fang Ou, Frederique Vanholsbeeck, Simon Swift","submitted_at":"2017-03-02T18:51:53Z","abstract_excerpt":"Aim: To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. Methods and Results: Mixtures of live and dead Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples were stained using SYTO 9 and propidium iodide and 6 {\\mu}m reference beads were added. Bacteria present in live samples were enumerated by agar plate counting. Bacteria present in dead samples were enumerated by agar plate counting before treatment with isopropanol. There is a linear relationship between the "},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1703.00895","kind":"arxiv","version":2},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.QM","submitted_at":"2017-03-02T18:51:53Z","cross_cats_sorted":["physics.bio-ph"],"title_canon_sha256":"c04e1bd8b9dcfb510b787676836152a10e4c6f6227887f6a9e5d91ea8f549090","abstract_canon_sha256":"9c721c0501faef91ee77c3070e6da678f4477f9a1e57412721afee95a63a626d"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-17T23:55:43.362714Z","signature_b64":"/Tgag+JKjplX7AO8yPsWd5OY/JoCYBHfGgCey+shocaIDZO4gbCmRVEPUqeHkHfqUp/7Wx+MWN7ltl2IZgkoCg==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"9c9c784c91a17ce78bebe59991f5b4ad2c57cabbc4173bfacd20d2c0a528c0f6","last_reissued_at":"2026-05-17T23:55:43.362053Z","signature_status":"signed_v1","first_computed_at":"2026-05-17T23:55:43.362053Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Absolute bacterial cell enumeration using flow cytometry","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["physics.bio-ph"],"primary_cat":"q-bio.QM","authors_text":"Cushla McGoverin, Fang Ou, Frederique Vanholsbeeck, Simon Swift","submitted_at":"2017-03-02T18:51:53Z","abstract_excerpt":"Aim: To evaluate a flow cytometry protocol that uses reference beads for the enumeration of live and dead bacteria present in a mixture. Methods and Results: Mixtures of live and dead Escherichia coli with live:dead concentration ratios varying from 0 to 100% were prepared. These samples were stained using SYTO 9 and propidium iodide and 6 {\\mu}m reference beads were added. Bacteria present in live samples were enumerated by agar plate counting. Bacteria present in dead samples were enumerated by agar plate counting before treatment with isopropanol. There is a linear relationship between the "},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1703.00895","kind":"arxiv","version":2},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1703.00895","created_at":"2026-05-17T23:55:43.362157+00:00"},{"alias_kind":"arxiv_version","alias_value":"1703.00895v2","created_at":"2026-05-17T23:55:43.362157+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1703.00895","created_at":"2026-05-17T23:55:43.362157+00:00"},{"alias_kind":"pith_short_12","alias_value":"TSOHQTERUF6O","created_at":"2026-05-18T12:31:46.661854+00:00"},{"alias_kind":"pith_short_16","alias_value":"TSOHQTERUF6OPC7L","created_at":"2026-05-18T12:31:46.661854+00:00"},{"alias_kind":"pith_short_8","alias_value":"TSOHQTER","created_at":"2026-05-18T12:31:46.661854+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU","json":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU.json","graph_json":"https://pith.science/api/pith-number/TSOHQTERUF6OPC7L4WMZD5NUVU/graph.json","events_json":"https://pith.science/api/pith-number/TSOHQTERUF6OPC7L4WMZD5NUVU/events.json","paper":"https://pith.science/paper/TSOHQTER"},"agent_actions":{"view_html":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU","download_json":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU.json","view_paper":"https://pith.science/paper/TSOHQTER","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1703.00895&json=true","fetch_graph":"https://pith.science/api/pith-number/TSOHQTERUF6OPC7L4WMZD5NUVU/graph.json","fetch_events":"https://pith.science/api/pith-number/TSOHQTERUF6OPC7L4WMZD5NUVU/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU/action/timestamp_anchor","attest_storage":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU/action/storage_attestation","attest_author":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU/action/author_attestation","sign_citation":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU/action/citation_signature","submit_replication":"https://pith.science/pith/TSOHQTERUF6OPC7L4WMZD5NUVU/action/replication_record"}},"created_at":"2026-05-17T23:55:43.362157+00:00","updated_at":"2026-05-17T23:55:43.362157+00:00"}