{"record_type":"pith_number_record","schema_url":"https://pith.science/schemas/pith-number/v1.json","pith_number":"pith:2017:XGVTAMK34S4DRK3VMNTCFAQOWF","short_pith_number":"pith:XGVTAMK3","schema_version":"1.0","canonical_sha256":"b9ab30315be4b838ab75636622820eb17f5d2e1132ff35b60d827117ca96ca28","source":{"kind":"arxiv","id":"1703.10346","version":1},"attestation_state":"computed","paper":{"title":"Experimental measurement of binding energy, selectivity and allostery using fluctuation theorems","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["cond-mat.soft","cond-mat.stat-mech","physics.chem-ph","q-bio.BM"],"primary_cat":"physics.bio-ph","authors_text":"Anna Alemany, Felix Ritort, Joan Camunas-Soler","submitted_at":"2017-03-30T08:10:22Z","abstract_excerpt":"Thermodynamic bulk measurements of binding reactions critically rely on the validity of the law of mass action and the assumption of a dilute solution. Yet important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not follow this fundamental law and require a particular reaction model. Here we introduce a fluctuation theorem for ligand binding and an experimental approach using single-molecule force-spectroscopy to determine binding energies, selectivity and allostery of nucleic acids, proteins and peptides in a model-independe"},"verification_status":{"content_addressed":true,"pith_receipt":true,"author_attested":false,"weak_author_claims":0,"strong_author_claims":0,"externally_anchored":false,"storage_verified":false,"citation_signatures":0,"replication_records":0,"graph_snapshot":true,"references_resolved":false,"formal_links_present":false},"canonical_record":{"source":{"id":"1703.10346","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"physics.bio-ph","submitted_at":"2017-03-30T08:10:22Z","cross_cats_sorted":["cond-mat.soft","cond-mat.stat-mech","physics.chem-ph","q-bio.BM"],"title_canon_sha256":"750eb1380228184d52bcb439ba4b7b0131ff40feab7a88064568cb1f8d32c6ca","abstract_canon_sha256":"4694ee53f6e4c5e3f368f99f8d0f6aa466d03d5da3bdceee29f5cfddfa7e11b4"},"schema_version":"1.0"},"receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T00:47:37.284119Z","signature_b64":"tqM5iJjTqCfh7hCDFr7oaRZUwcUBwLISBLGyO8lcsXLimZ2ABkFykBr4BoS/WovLXJ1HJRrsjGxpNpdaGHCqCA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"b9ab30315be4b838ab75636622820eb17f5d2e1132ff35b60d827117ca96ca28","last_reissued_at":"2026-05-18T00:47:37.283509Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T00:47:37.283509Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"graph_snapshot":{"paper":{"title":"Experimental measurement of binding energy, selectivity and allostery using fluctuation theorems","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":["cond-mat.soft","cond-mat.stat-mech","physics.chem-ph","q-bio.BM"],"primary_cat":"physics.bio-ph","authors_text":"Anna Alemany, Felix Ritort, Joan Camunas-Soler","submitted_at":"2017-03-30T08:10:22Z","abstract_excerpt":"Thermodynamic bulk measurements of binding reactions critically rely on the validity of the law of mass action and the assumption of a dilute solution. Yet important biological systems such as allosteric ligand-receptor binding, macromolecular crowding, or misfolded molecules may not follow this fundamental law and require a particular reaction model. Here we introduce a fluctuation theorem for ligand binding and an experimental approach using single-molecule force-spectroscopy to determine binding energies, selectivity and allostery of nucleic acids, proteins and peptides in a model-independe"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1703.10346","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"aliases":[{"alias_kind":"arxiv","alias_value":"1703.10346","created_at":"2026-05-18T00:47:37.283606+00:00"},{"alias_kind":"arxiv_version","alias_value":"1703.10346v1","created_at":"2026-05-18T00:47:37.283606+00:00"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1703.10346","created_at":"2026-05-18T00:47:37.283606+00:00"},{"alias_kind":"pith_short_12","alias_value":"XGVTAMK34S4D","created_at":"2026-05-18T12:31:53.515858+00:00"},{"alias_kind":"pith_short_16","alias_value":"XGVTAMK34S4DRK3V","created_at":"2026-05-18T12:31:53.515858+00:00"},{"alias_kind":"pith_short_8","alias_value":"XGVTAMK3","created_at":"2026-05-18T12:31:53.515858+00:00"}],"events":[],"event_summary":{},"paper_claims":[],"inbound_citations":{"count":0,"internal_anchor_count":0,"sample":[]},"formal_canon":{"evidence_count":0,"sample":[],"anchors":[]},"links":{"html":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF","json":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF.json","graph_json":"https://pith.science/api/pith-number/XGVTAMK34S4DRK3VMNTCFAQOWF/graph.json","events_json":"https://pith.science/api/pith-number/XGVTAMK34S4DRK3VMNTCFAQOWF/events.json","paper":"https://pith.science/paper/XGVTAMK3"},"agent_actions":{"view_html":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF","download_json":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF.json","view_paper":"https://pith.science/paper/XGVTAMK3","resolve_alias":"https://pith.science/api/pith-number/resolve?arxiv=1703.10346&json=true","fetch_graph":"https://pith.science/api/pith-number/XGVTAMK34S4DRK3VMNTCFAQOWF/graph.json","fetch_events":"https://pith.science/api/pith-number/XGVTAMK34S4DRK3VMNTCFAQOWF/events.json","actions":{"anchor_timestamp":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF/action/timestamp_anchor","attest_storage":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF/action/storage_attestation","attest_author":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF/action/author_attestation","sign_citation":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF/action/citation_signature","submit_replication":"https://pith.science/pith/XGVTAMK34S4DRK3VMNTCFAQOWF/action/replication_record"}},"created_at":"2026-05-18T00:47:37.283606+00:00","updated_at":"2026-05-18T00:47:37.283606+00:00"}