{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2025:XR7RMQQXR2767UYWWLR7JMZIOD","short_pith_number":"pith:XR7RMQQX","canonical_record":{"source":{"id":"2504.02969","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"physics.optics","submitted_at":"2025-04-03T18:45:01Z","cross_cats_sorted":["physics.app-ph","physics.bio-ph"],"title_canon_sha256":"2e2aae234200385df7a6be3ee973ae3900a451157f72aef8c60524d7fdd04360","abstract_canon_sha256":"085f3226967a021e388f403906a5568e2b8509df97001c5549cb35a9fd54a6a7"},"schema_version":"1.0"},"canonical_sha256":"bc7f1642178ebfefd316b2e3f4b32870ed09f051d7581db99e6dadc8d335163d","source":{"kind":"arxiv","id":"2504.02969","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2504.02969","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"arxiv_version","alias_value":"2504.02969v1","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2504.02969","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_12","alias_value":"XR7RMQQXR276","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_16","alias_value":"XR7RMQQXR2767UYW","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_8","alias_value":"XR7RMQQX","created_at":"2026-07-05T10:44:05Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2025:XR7RMQQXR2767UYWWLR7JMZIOD","target":"record","payload":{"canonical_record":{"source":{"id":"2504.02969","kind":"arxiv","version":1},"metadata":{"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"physics.optics","submitted_at":"2025-04-03T18:45:01Z","cross_cats_sorted":["physics.app-ph","physics.bio-ph"],"title_canon_sha256":"2e2aae234200385df7a6be3ee973ae3900a451157f72aef8c60524d7fdd04360","abstract_canon_sha256":"085f3226967a021e388f403906a5568e2b8509df97001c5549cb35a9fd54a6a7"},"schema_version":"1.0"},"canonical_sha256":"bc7f1642178ebfefd316b2e3f4b32870ed09f051d7581db99e6dadc8d335163d","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-07-05T10:44:05.837172Z","signature_b64":"1yRZ8GAtVziOI+Bigmz9xACV3TAJIXhZM2IQ2G1Cgx02CqxSVcrbECE34sOmliLiiSRHlNBL3iYgc9PAnoR8CA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"bc7f1642178ebfefd316b2e3f4b32870ed09f051d7581db99e6dadc8d335163d","last_reissued_at":"2026-07-05T10:44:05.836704Z","signature_status":"signed_v1","first_computed_at":"2026-07-05T10:44:05.836704Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"2504.02969","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T10:44:05Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"YgzO5QI6W26gVmz8SOdx+qgSxZ0/WMbDR1fnMoW8zNHqk9wUOmO2h+afwbMleg0wX1GK7nOiVwR9vTp1WUGJCw==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-03T16:09:29.416762Z"},"content_sha256":"402cba1fee6b027fcd0f30bdf2dd81a728a2ca3801d9d385de281c795c141fdb","schema_version":"1.0","event_id":"sha256:402cba1fee6b027fcd0f30bdf2dd81a728a2ca3801d9d385de281c795c141fdb"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2025:XR7RMQQXR2767UYWWLR7JMZIOD","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"Thermal Lensing Effects in Two-Photon Light-Sheet Microscopy","license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","headline":"","cross_cats":["physics.app-ph","physics.bio-ph"],"primary_cat":"physics.optics","authors_text":"Antoine Hubert, Georges Debr\\'egeas, Hugo Trentesaux, Thomas Pujol, Volker Bormuth","submitted_at":"2025-04-03T18:45:01Z","abstract_excerpt":"In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging performance. Two-photon LSFM (2P-LSFM), in particular, improves the signal-to-background ratio by reducing laser scattering and distortion in biological specimens. However, we report a potentially detrimental thermal effect in 2P-LSFM: the high laser powers required for two-photon excitation induce localized heating, which alters the refractive index of the medium and effectively forms a divergent the"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2504.02969","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"integrity":{"clean":true,"summary":{"advisory":0,"critical":0,"by_detector":{},"informational":0},"endpoint":"/pith/2504.02969/integrity.json","findings":[],"available":true,"detectors_run":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938"},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-07-05T10:44:05Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"cVhfxhVAohjf5A79hdxMqVpwfwU6BXj8/PLGLuDJKOTN8w6IWU/ZBuB4awjSk6U6jvzjGFJEUqGDgnxJt/3oDg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-08-03T16:09:29.417252Z"},"content_sha256":"074be18b64f70e6d00a1e8bb907149b400f4adf6549a3119731b4c3d06e4aee2","schema_version":"1.0","event_id":"sha256:074be18b64f70e6d00a1e8bb907149b400f4adf6549a3119731b4c3d06e4aee2"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/XR7RMQQXR2767UYWWLR7JMZIOD/bundle.json","state_url":"https://pith.science/pith/XR7RMQQXR2767UYWWLR7JMZIOD/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/XR7RMQQXR2767UYWWLR7JMZIOD/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-08-03T16:09:29Z","links":{"resolver":"https://pith.science/pith/XR7RMQQXR2767UYWWLR7JMZIOD","bundle":"https://pith.science/pith/XR7RMQQXR2767UYWWLR7JMZIOD/bundle.json","state":"https://pith.science/pith/XR7RMQQXR2767UYWWLR7JMZIOD/state.json","well_known_bundle":"https://pith.science/.well-known/pith/XR7RMQQXR2767UYWWLR7JMZIOD/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2025:XR7RMQQXR2767UYWWLR7JMZIOD","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"085f3226967a021e388f403906a5568e2b8509df97001c5549cb35a9fd54a6a7","cross_cats_sorted":["physics.app-ph","physics.bio-ph"],"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"physics.optics","submitted_at":"2025-04-03T18:45:01Z","title_canon_sha256":"2e2aae234200385df7a6be3ee973ae3900a451157f72aef8c60524d7fdd04360"},"schema_version":"1.0","source":{"id":"2504.02969","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"2504.02969","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"arxiv_version","alias_value":"2504.02969v1","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.2504.02969","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_12","alias_value":"XR7RMQQXR276","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_16","alias_value":"XR7RMQQXR2767UYW","created_at":"2026-07-05T10:44:05Z"},{"alias_kind":"pith_short_8","alias_value":"XR7RMQQX","created_at":"2026-07-05T10:44:05Z"}],"graph_snapshots":[{"event_id":"sha256:074be18b64f70e6d00a1e8bb907149b400f4adf6549a3119731b4c3d06e4aee2","target":"graph","created_at":"2026-07-05T10:44:05Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"integrity":{"available":true,"clean":true,"detectors_run":[],"endpoint":"/pith/2504.02969/integrity.json","findings":[],"snapshot_sha256":"c28c3603d3b5d939e8dc4c7e95fa8dfce3d595e45f758748cecf8e644a296938","summary":{"advisory":0,"by_detector":{},"critical":0,"informational":0}},"paper":{"abstract_excerpt":"In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging performance. Two-photon LSFM (2P-LSFM), in particular, improves the signal-to-background ratio by reducing laser scattering and distortion in biological specimens. However, we report a potentially detrimental thermal effect in 2P-LSFM: the high laser powers required for two-photon excitation induce localized heating, which alters the refractive index of the medium and effectively forms a divergent the","authors_text":"Antoine Hubert, Georges Debr\\'egeas, Hugo Trentesaux, Thomas Pujol, Volker Bormuth","cross_cats":["physics.app-ph","physics.bio-ph"],"headline":"","license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"physics.optics","submitted_at":"2025-04-03T18:45:01Z","title":"Thermal Lensing Effects in Two-Photon Light-Sheet Microscopy"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"2504.02969","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:402cba1fee6b027fcd0f30bdf2dd81a728a2ca3801d9d385de281c795c141fdb","target":"record","created_at":"2026-07-05T10:44:05Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"085f3226967a021e388f403906a5568e2b8509df97001c5549cb35a9fd54a6a7","cross_cats_sorted":["physics.app-ph","physics.bio-ph"],"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","primary_cat":"physics.optics","submitted_at":"2025-04-03T18:45:01Z","title_canon_sha256":"2e2aae234200385df7a6be3ee973ae3900a451157f72aef8c60524d7fdd04360"},"schema_version":"1.0","source":{"id":"2504.02969","kind":"arxiv","version":1}},"canonical_sha256":"bc7f1642178ebfefd316b2e3f4b32870ed09f051d7581db99e6dadc8d335163d","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"bc7f1642178ebfefd316b2e3f4b32870ed09f051d7581db99e6dadc8d335163d","first_computed_at":"2026-07-05T10:44:05.836704Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-07-05T10:44:05.836704Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"1yRZ8GAtVziOI+Bigmz9xACV3TAJIXhZM2IQ2G1Cgx02CqxSVcrbECE34sOmliLiiSRHlNBL3iYgc9PAnoR8CA==","signature_status":"signed_v1","signed_at":"2026-07-05T10:44:05.837172Z","signed_message":"canonical_sha256_bytes"},"source_id":"2504.02969","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:402cba1fee6b027fcd0f30bdf2dd81a728a2ca3801d9d385de281c795c141fdb","sha256:074be18b64f70e6d00a1e8bb907149b400f4adf6549a3119731b4c3d06e4aee2"],"state_sha256":"c0a5151521c003e2949d5a89c950e03e6b5d330311ec13a90bf1cb2d9fc11ec3"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"0w2pYaBQIkrX9J5ikg/3QnBL6doWH+hMODm97Gt8HMsu0hBAgrgcGJYQzhc6mMpZFd/XoxhFGePZEbAV9Ej9Bg==","signed_message":"bundle_sha256_bytes","signed_at":"2026-08-03T16:09:29.422182Z","bundle_sha256":"9ddde0f5aa348be3e9ee4b782403eaefa9a2da5b2f8d33df680ceda7b739d3de"}}