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Cavity ring-down direct absorption spectra and astronomical observations of HD 183143 with the HERMES spectrograph on the Mercator Telescope in La Palma and the McKellar spectrograph on the DAO 1.2 m Telescope are compared. In the 543-547 nm region a broad band is measured with a band maximum at 545 nm and FWHM of 1.03(0.1) nm coinciding with a well-known diffuse interstellar band at lambda 5450 with FWHM of 0.953 nm. 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