{"bundle_type":"pith_open_graph_bundle","bundle_version":"1.0","pith_number":"pith:2012:YNPOJIHF6LGJ3DRGVB2ZCPJGI5","short_pith_number":"pith:YNPOJIHF","canonical_record":{"source":{"id":"1207.1957","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-07-09T06:18:53Z","cross_cats_sorted":[],"title_canon_sha256":"d5058ecaf0df9b2677e6b931c765d486319c977809dea68d202835c396c0d765","abstract_canon_sha256":"70e5002cb8b9350007a134f20d7ac4cab9bdaefc0c760fe8240d67dd3fc48f3d"},"schema_version":"1.0"},"canonical_sha256":"c35ee4a0e5f2cc9d8e26a875913d2647765296cfbb60aa256a69e8631247f932","source":{"kind":"arxiv","id":"1207.1957","version":1},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1207.1957","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"arxiv_version","alias_value":"1207.1957v1","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1207.1957","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"pith_short_12","alias_value":"YNPOJIHF6LGJ","created_at":"2026-05-18T12:27:27Z"},{"alias_kind":"pith_short_16","alias_value":"YNPOJIHF6LGJ3DRG","created_at":"2026-05-18T12:27:27Z"},{"alias_kind":"pith_short_8","alias_value":"YNPOJIHF","created_at":"2026-05-18T12:27:27Z"}],"events":[{"event_type":"record_created","subject_pith_number":"pith:2012:YNPOJIHF6LGJ3DRGVB2ZCPJGI5","target":"record","payload":{"canonical_record":{"source":{"id":"1207.1957","kind":"arxiv","version":1},"metadata":{"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-07-09T06:18:53Z","cross_cats_sorted":[],"title_canon_sha256":"d5058ecaf0df9b2677e6b931c765d486319c977809dea68d202835c396c0d765","abstract_canon_sha256":"70e5002cb8b9350007a134f20d7ac4cab9bdaefc0c760fe8240d67dd3fc48f3d"},"schema_version":"1.0"},"canonical_sha256":"c35ee4a0e5f2cc9d8e26a875913d2647765296cfbb60aa256a69e8631247f932","receipt":{"kind":"pith_receipt","key_id":"pith-v1-2026-05","algorithm":"ed25519","signed_at":"2026-05-18T03:51:26.492251Z","signature_b64":"rB8/do1sFCI5fCfEAOiKWO/OSlZ6qKlgxFp1xhwyMV2lgpxcJmwHAy9LuO89zVVwKzqjeRBwDZmNe9Gbgwh+DA==","signed_message":"canonical_sha256_bytes","builder_version":"pith-number-builder-2026-05-17-v1","receipt_version":"0.3","canonical_sha256":"c35ee4a0e5f2cc9d8e26a875913d2647765296cfbb60aa256a69e8631247f932","last_reissued_at":"2026-05-18T03:51:26.491605Z","signature_status":"signed_v1","first_computed_at":"2026-05-18T03:51:26.491605Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"source_kind":"arxiv","source_id":"1207.1957","source_version":1,"attestation_state":"computed"},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:51:26Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"MYHXfdbN1tvXCiIZ9JL+HqIxsZlpGe2NaHiiQsmRw2TCJo830Iu7iKVPXp1N4nLAxbXOB/GDW9kJOYJA7gmwAg==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-27T18:10:03.151489Z"},"content_sha256":"2dccd3f25fa09fb88e0f6489d411b4446aa5a2df934b0786d0a46213a756fb91","schema_version":"1.0","event_id":"sha256:2dccd3f25fa09fb88e0f6489d411b4446aa5a2df934b0786d0a46213a756fb91"},{"event_type":"graph_snapshot","subject_pith_number":"pith:2012:YNPOJIHF6LGJ3DRGVB2ZCPJGI5","target":"graph","payload":{"graph_snapshot":{"paper":{"title":"About the mechanism of interference of silver staining with peptide mass spectrometry","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","headline":"","cross_cats":[],"primary_cat":"q-bio.GN","authors_text":"Alain Van Dorsselaer (IPHC), Emmanuelle Leize-Wagner (IPHC), LCBM), Mireille Chevallet (BECP), Sophie Richert (IPHC), Sylvie Luche (BECP), Thierry Rabilloud (BECP","submitted_at":"2012-07-09T06:18:53Z","abstract_excerpt":"The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining development in this interference process. Consequently, a formaldehyde-free staining protocol has been devised, using carbohydrazide as the developing agent. This protocol showed much increased peptide coverage"},"claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1207.1957","kind":"arxiv","version":1},"verdict":{"id":null,"model_set":{},"created_at":null,"strongest_claim":"","one_line_summary":"","pipeline_version":null,"weakest_assumption":"","pith_extraction_headline":""},"references":{"count":0,"sample":[],"resolved_work":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","internal_anchors":0},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"author_claims":{"count":0,"strong_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"builder_version":"pith-number-builder-2026-05-17-v1"},"verdict_id":null},"signer":{"signer_id":"pith.science","signer_type":"pith_registry","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54"},"created_at":"2026-05-18T03:51:26Z","supersedes":[],"prev_event":null,"signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"GN3hX8VjZ0Xc9hdYiQO14WoQLK7BKreoV7nivxyO9ZX66JHxlMhvOJqVJ/vC8TWiOHiUCyEUWmwNP3f4W86QBA==","signed_message":"open_graph_event_sha256_bytes","signed_at":"2026-06-27T18:10:03.151841Z"},"content_sha256":"5facb59d111df24207980e7f2bdce52c0d1cf07a3d3cae8385ec38f0b4351b3a","schema_version":"1.0","event_id":"sha256:5facb59d111df24207980e7f2bdce52c0d1cf07a3d3cae8385ec38f0b4351b3a"}],"timestamp_proofs":[],"mirror_hints":[{"mirror_type":"https","name":"Pith Resolver","base_url":"https://pith.science","bundle_url":"https://pith.science/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/bundle.json","state_url":"https://pith.science/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/state.json","well_known_bundle_url":"https://pith.science/.well-known/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/bundle.json","status":"primary"}],"public_keys":[{"key_id":"pith-v1-2026-05","algorithm":"ed25519","format":"raw","public_key_b64":"stVStoiQhXFxp4s2pdzPNoqVNBMojDU/fJ2db5S3CbM=","public_key_hex":"b2d552b68890857171a78b36a5dccf368a953413288c353f7c9d9d6f94b709b3","fingerprint_sha256_b32_first128bits":"RVFV5Z2OI2J3ZUO7ERDEBCYNKS","fingerprint_sha256_hex":"8d4b5ee74e4693bcd1df2446408b0d54","rotates_at":null,"url":"https://pith.science/pith-signing-key.json","notes":"Pith uses this Ed25519 key to sign canonical record SHA-256 digests. Verify with: ed25519_verify(public_key, message=canonical_sha256_bytes, signature=base64decode(signature_b64))."}],"merge_version":"pith-open-graph-merge-v1","built_at":"2026-06-27T18:10:03Z","links":{"resolver":"https://pith.science/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5","bundle":"https://pith.science/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/bundle.json","state":"https://pith.science/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/state.json","well_known_bundle":"https://pith.science/.well-known/pith/YNPOJIHF6LGJ3DRGVB2ZCPJGI5/bundle.json"},"state":{"state_type":"pith_open_graph_state","state_version":"1.0","pith_number":"pith:2012:YNPOJIHF6LGJ3DRGVB2ZCPJGI5","merge_version":"pith-open-graph-merge-v1","event_count":2,"valid_event_count":2,"invalid_event_count":0,"equivocation_count":0,"current":{"canonical_record":{"metadata":{"abstract_canon_sha256":"70e5002cb8b9350007a134f20d7ac4cab9bdaefc0c760fe8240d67dd3fc48f3d","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-07-09T06:18:53Z","title_canon_sha256":"d5058ecaf0df9b2677e6b931c765d486319c977809dea68d202835c396c0d765"},"schema_version":"1.0","source":{"id":"1207.1957","kind":"arxiv","version":1}},"source_aliases":[{"alias_kind":"arxiv","alias_value":"1207.1957","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"arxiv_version","alias_value":"1207.1957v1","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"doi","alias_value":"10.48550/arxiv.1207.1957","created_at":"2026-05-18T03:51:26Z"},{"alias_kind":"pith_short_12","alias_value":"YNPOJIHF6LGJ","created_at":"2026-05-18T12:27:27Z"},{"alias_kind":"pith_short_16","alias_value":"YNPOJIHF6LGJ3DRG","created_at":"2026-05-18T12:27:27Z"},{"alias_kind":"pith_short_8","alias_value":"YNPOJIHF","created_at":"2026-05-18T12:27:27Z"}],"graph_snapshots":[{"event_id":"sha256:5facb59d111df24207980e7f2bdce52c0d1cf07a3d3cae8385ec38f0b4351b3a","target":"graph","created_at":"2026-05-18T03:51:26Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"graph_snapshot":{"author_claims":{"count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57","strong_count":0},"builder_version":"pith-number-builder-2026-05-17-v1","claims":{"count":0,"items":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"formal_canon":{"evidence_count":0,"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"paper":{"abstract_excerpt":"The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining development in this interference process. Consequently, a formaldehyde-free staining protocol has been devised, using carbohydrazide as the developing agent. This protocol showed much increased peptide coverage","authors_text":"Alain Van Dorsselaer (IPHC), Emmanuelle Leize-Wagner (IPHC), LCBM), Mireille Chevallet (BECP), Sophie Richert (IPHC), Sylvie Luche (BECP), Thierry Rabilloud (BECP","cross_cats":[],"headline":"","license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-07-09T06:18:53Z","title":"About the mechanism of interference of silver staining with peptide mass spectrometry"},"references":{"count":0,"internal_anchors":0,"resolved_work":0,"sample":[],"snapshot_sha256":"258153158e38e3291e3d48162225fcdb2d5a3ed65a07baac614ab91432fd4f57"},"source":{"id":"1207.1957","kind":"arxiv","version":1},"verdict":{"created_at":null,"id":null,"model_set":{},"one_line_summary":"","pipeline_version":null,"pith_extraction_headline":"","strongest_claim":"","weakest_assumption":""}},"verdict_id":null}}],"author_attestations":[],"timestamp_anchors":[],"storage_attestations":[],"citation_signatures":[],"replication_records":[],"corrections":[],"mirror_hints":[],"record_created":{"event_id":"sha256:2dccd3f25fa09fb88e0f6489d411b4446aa5a2df934b0786d0a46213a756fb91","target":"record","created_at":"2026-05-18T03:51:26Z","signer":{"key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signer_id":"pith.science","signer_type":"pith_registry"},"payload":{"attestation_state":"computed","canonical_record":{"metadata":{"abstract_canon_sha256":"70e5002cb8b9350007a134f20d7ac4cab9bdaefc0c760fe8240d67dd3fc48f3d","cross_cats_sorted":[],"license":"http://arxiv.org/licenses/nonexclusive-distrib/1.0/","primary_cat":"q-bio.GN","submitted_at":"2012-07-09T06:18:53Z","title_canon_sha256":"d5058ecaf0df9b2677e6b931c765d486319c977809dea68d202835c396c0d765"},"schema_version":"1.0","source":{"id":"1207.1957","kind":"arxiv","version":1}},"canonical_sha256":"c35ee4a0e5f2cc9d8e26a875913d2647765296cfbb60aa256a69e8631247f932","receipt":{"algorithm":"ed25519","builder_version":"pith-number-builder-2026-05-17-v1","canonical_sha256":"c35ee4a0e5f2cc9d8e26a875913d2647765296cfbb60aa256a69e8631247f932","first_computed_at":"2026-05-18T03:51:26.491605Z","key_id":"pith-v1-2026-05","kind":"pith_receipt","last_reissued_at":"2026-05-18T03:51:26.491605Z","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","receipt_version":"0.3","signature_b64":"rB8/do1sFCI5fCfEAOiKWO/OSlZ6qKlgxFp1xhwyMV2lgpxcJmwHAy9LuO89zVVwKzqjeRBwDZmNe9Gbgwh+DA==","signature_status":"signed_v1","signed_at":"2026-05-18T03:51:26.492251Z","signed_message":"canonical_sha256_bytes"},"source_id":"1207.1957","source_kind":"arxiv","source_version":1}}},"equivocations":[],"invalid_events":[],"applied_event_ids":["sha256:2dccd3f25fa09fb88e0f6489d411b4446aa5a2df934b0786d0a46213a756fb91","sha256:5facb59d111df24207980e7f2bdce52c0d1cf07a3d3cae8385ec38f0b4351b3a"],"state_sha256":"945bd1adcf314520de570a3b7d6c9e2fa193f178c6b45a5e17abdef7b12fd61d"},"bundle_signature":{"signature_status":"signed_v1","algorithm":"ed25519","key_id":"pith-v1-2026-05","public_key_fingerprint":"8d4b5ee74e4693bcd1df2446408b0d54","signature_b64":"2yT2uTuy1Qb0fulMbe5OgZnxJ45Yg5BZSl2r92g0VOP2Hr6LiIC35NHKWdHYRSuTMy2X99BttorcGhA7oh2MCg==","signed_message":"bundle_sha256_bytes","signed_at":"2026-06-27T18:10:03.153777Z","bundle_sha256":"abc5b6759a9cc6bf7b5281ad75e30c1b58553744030f29c6e3a0c69f49ec8bdb"}}