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In the Coulomb branch, the instanton corrects both the MHV and next-to-next-MHV higher derivative terms D^4F^{2n+2} and F^{2n+4}. We confirm at the non-perturbative level the non-renormalization theorems for MHV F^{2n+2} terms that are expected to receive perturbative corrections only at n-loops. 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