Not yet reviewed by Pith; the record is open.

We investigate the effects of crowding on the conformations and assembly of confined, highly charged, and thick polyelectrolyte brushes in the osmotic regime. Particle tracking experiments on increasingly dense suspensions of colloids coated with ultra-long double stranded DNA (dsDNA) fragments reveal non-monotonic particle shrinking, aggregation and re-entrant ordering. Theory and simulations show that shrinking is induced by the osmotic pressure exerted by the counterions absorbed in neighbor brushes, while aggregation and re-entrant ordering are the effect of a short-range attraction competing with the electrostatic repulsion. Blunt-end interactions between dsDNA fragments of neighboring brushes are responsible for the attraction and can be tuned by inducing free-end backfolding through the addition of monovalent salt. Our results show that base stacking is a mode parallel to hybridization to steer colloidal assembly, in which attractions can be fine-tuned through salinity and, potentially, grafting density and temperature.