A relay dye increases the energy transfer efficiency from donor to acceptor in a cascaded three-dye FRET system compared to a direct two-dye system.
Förster, Discussions Faraday Soc., 27 (1959) 7 - 17
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Cascaded F\"orster Resonance Energy Transfer and Role of the Relay Dye
A relay dye increases the energy transfer efficiency from donor to acceptor in a cascaded three-dye FRET system compared to a direct two-dye system.