In tapping-mode AFM, feedback-induced forces on fragile molecules are minimized by exciting at the lower resonance-slope frequency, where the force-to-interaction-depth coefficient drops to about 0.3 times the cantilever spring constant for a quality factor of 1.5.
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Guidelines for Fast and Nondestructive Imaging in AM-AFM
In tapping-mode AFM, feedback-induced forces on fragile molecules are minimized by exciting at the lower resonance-slope frequency, where the force-to-interaction-depth coefficient drops to about 0.3 times the cantilever spring constant for a quality factor of 1.5.