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arxiv: 1007.0471 · v1 · submitted 2010-07-03 · 🧬 q-bio.OT

Technical performance and interpretation of physical experiment in problems of cell biology

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keywords cellexperimentexperimentscellsdesigninterpretationintroduceliving
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The lecture summarises main results of my team over last five years in the field of technical experiment design and interpretation of results of experiments for cell bi-ology. I introduce the theoretical concept of the experiment, based mainly on ideqas of stochastic systems theory, and confront it with general ideas of systems theory. In the next part I introduce available experiments and discuss their information con-tent. Namely, I show that light microscopy may be designed to give resolution com-parable to that of electron microscopy and that may be used for experiments using living cells. I show avenues to objective analysis of cell behavior observation. I pro-pose new microscope design, which shall combine advantages of all methods, and steps to be taken to build a model of living cells with predictive power for practical use

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