Pith Number

pith:WRRIUJJT

pith:2025:WRRIUJJT2RGK27H77BQU6N5R4S

not attested not anchored not stored refs pending

MOSAIC: Codon Harmonization of Monte Carlo-Based Simulated Annealing for Linked Codons in Heterologous Protein Expression

Chengcheng Yang, Eok Kyun Lee, Insung S. Choi, Jihoo Kim, Ryan Fernandez Medina Hariri, Seung Seo Lee, Won June Kim, Yoonho Jeong, Younghoon Lee

A Monte Carlo-based algorithm for harmonizing linked codon sets improves heterologous expression of rate-sensitive proteins like ribosomal S18.

arxiv:2511.10708 v4 · 2025-11-13 · q-bio.QM

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\usepackage{pith}
\pithnumber{WRRIUJJT2RGK27H77BQU6N5R4S}

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Record completeness

1 Bitcoin timestamp

2 Internet Archive

3 Author claim open · sign in to claim

4 Citations open

5 Replications open

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The bundle contains the canonical record plus signed events. A mirror can host it anywhere and recompute the same current state with the deterministic merge algorithm.

Claims

C1strongest claim

The harmonized gene of RP S18 was expressed and compared with the expression of the wild-type gene. The harmonized gene clearly yielded a larger quantity of the protein, from which the amount of the soluble protein was also significant.

C2weakest assumption

That harmonizing linked codon sets will replicate native translation speeds and thereby preserve proper co-translational folding for proteins sensitive to translation rates, without introducing new folding problems.

C3one line summary

MOSAIC applies simulated annealing to harmonize linked codon sets, yielding higher total and soluble protein levels for ribosomal protein S18 versus wild-type in heterologous expression.

Formal links

2 machine-checked theorem links

Receipt and verification

First computed	2026-05-20T00:00:27.929635Z
Builder	pith-number-builder-2026-05-17-v1
Signature	Pith Ed25519 (`pith-v1-2026-05`) · public key
Schema	pith-number/v1.0

Canonical hash

b4628a2533d44cad7cfff8614f37b1e48df763438ee6827814affa61d161ce86

Aliases

arxiv: 2511.10708 · arxiv_version: 2511.10708v4 · doi: 10.48550/arxiv.2511.10708 · pith_short_12: WRRIUJJT2RGK · pith_short_16: WRRIUJJT2RGK27H7 · pith_short_8: WRRIUJJT

Agent API

Resolver JSON Graph JSON Events JSON Schema Signing key

Verify this Pith Number yourself

curl -sH 'Accept: application/ld+json' https://pith.science/pith/WRRIUJJT2RGK27H77BQU6N5R4S \
  | jq -c '.canonical_record' \
  | python3 -c "import sys,json,hashlib; b=json.dumps(json.loads(sys.stdin.read()), sort_keys=True, separators=(',',':'), ensure_ascii=False).encode(); print(hashlib.sha256(b).hexdigest())"
# expect: b4628a2533d44cad7cfff8614f37b1e48df763438ee6827814affa61d161ce86

Canonical record JSON

{
  "metadata": {
    "abstract_canon_sha256": "54fe97dbe07878b57b7320f108fcf6fcbe0e49a607e80e761efedaf1feea64cd",
    "cross_cats_sorted": [],
    "license": "http://arxiv.org/licenses/nonexclusive-distrib/1.0/",
    "primary_cat": "q-bio.QM",
    "submitted_at": "2025-11-13T07:07:32Z",
    "title_canon_sha256": "8536320d372817869fb58d9e3b757d93a730bfc429af13f40a387c94af919231"
  },
  "schema_version": "1.0",
  "source": {
    "id": "2511.10708",
    "kind": "arxiv",
    "version": 4
  }
}