Urea induces a concentration-dependent dehydration-rehydration cycle on the BSA surface, with protein-water hydrogen bonds replaced by protein-urea bonds at low concentrations and partially restored as urea self-aggregates at higher concentrations.
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Molecular Mechanisms Underlying the Effects of Urea and the Structural Dynamics of Bovine Serum Albumin
Urea induces a concentration-dependent dehydration-rehydration cycle on the BSA surface, with protein-water hydrogen bonds replaced by protein-urea bonds at low concentrations and partially restored as urea self-aggregates at higher concentrations.