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arxiv: 0712.2919 · v1 · submitted 2007-12-18 · ⚛️ physics.bio-ph

DNA ejection from bacteriophage: towards a general behavior for osmotic suppression experiments

classification ⚛️ physics.bio-ph
keywords ableaccountanalyticalanalyzedbacteriophagebacteriophagesbehaviorbending
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We present in this work in vitro measurements of the force ejecting DNA from two distinct bacteriophages (T5 and lambda) using the smotic-suppression technique. Our data are analyzed by revisiting the current theories of DNA packaging in spherical capsids. In particular we show that a simplified analytical model based on bending considerations only is able to account quantitatively for the experimental findings. Physical and biological consequences are discussed.

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