Pitfalls in the quantitative analysis of random walks and the mapping of single-molecule dynamics at the cellular scale

In recent years Bayesian Inference has become an efficient tool to analyse single molecule trajectories. Recently, high density single molecule tagging, Langevin Equation modelling and Bayesian Inference [10] have been used to infer diffusion, force and potential fields at the full cell scale. In this short comment, we point out pitfalls [1, 2] to avoid in single molecule analysis in order to get unbiased results and reliable fields at various scales.