CKM matrix elements from tree-level decays and b-hadron lifetimes

We give an updated summary of the topics covered in Working Group I of the 2nd Workshop on the CKM Unitarity Triangle, with emphasis on the results obtained since the 1st CKM Workshop. The topics covered include the measurement of |V_{ub}|, of |V_{cb}| and of non-perturbative Heavy Quark Expansion parameters, and the determination of b-hadron lifetimes and lifetime differences.