Characterizing and tracking single colloidal particles with video holographic microscopy
classification
⚛️ physics.optics
cond-mat.soft
keywords
colloidalholographicmeasuremicroscopynanometerparticlessimultaneouslyaxial
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We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.
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