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Precise characterization of nanometer-scale systems using interferometric scattering microscopy and Bayesian analysis
Optics · Submitted 4 Jul 2023
abstract compiled · 2026-09-23 · no language-model review
The authors' main claim We apply the method to track a diffusing particle in three dimensions, to directly infer the diffusion coefficient of a nanoparticle without calculating a mean-square displacement, and to quantify the ejection of DNA from an individual lambda phage virus, demonstrating that the approach can be used to infer both static and dynamic properties of nanoscale systems.
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The abstract, sentence by sentence
Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of measurements. We demonstrate an alternative approach: modeling the interferometric point spread function (iPSF) and fitting this model to data within a Bayesian framework. This approach yields best-fit parameters, including the particle's three-dimensional position and polarizability, as well as uncertainties and correlations between these parameters. Building on recent work, we develop a model that is parameterized for rapid fitting. The model is designed to work with Hamiltonian Monte Carlo techniques that leverage automatic differentiation. We validate this approach by fitting the model to interferometric images of colloidal nanoparticles. We apply the method to track a diffusing particle in three dimensions, to directly infer the diffusion coefficient of a nanoparticle without calculating a mean-square displacement, and to quantify the ejection of DNA from an individual lambda phage virus, demonstrating that the approach can be used to infer both static and dynamic properties of nanoscale systems.
background contribution method result interpretation main claim
| # | sentence | role | the authors' own work | states the main conclusion | strength |
|---|---|---|---|---|---|
| s0 | Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. | background 1.00 | 0.09 | 0.03 | asserted 0.91 |
| s1 | The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of measurements. | background 0.71 | 0.08 | 0.02 | asserted 1.00 |
| s2 | We demonstrate an alternative approach: modeling the interferometric point spread function (iPSF) and fitting this model to data within a Bayesian framework. | method 0.60 | 0.92 | 0.11 | asserted 1.00 |
| s3 | This approach yields best-fit parameters, including the particle's three-dimensional position and polarizability, as well as uncertainties and correlations between these parameters. | result 0.51 | 0.86 | 0.18 | asserted 1.00 |
| s4 | Building on recent work, we develop a model that is parameterized for rapid fitting. | method 0.82 | 0.89 | 0.03 | asserted 1.00 |
| s5 | The model is designed to work with Hamiltonian Monte Carlo techniques that leverage automatic differentiation. | method 0.99 | 0.77 | 0.03 | asserted 1.00 |
| s6 | We validate this approach by fitting the model to interferometric images of colloidal nanoparticles. | method 0.80 | 0.96 | 0.05 | asserted 1.00 |
| s7 | We apply the method to track a diffusing particle in three dimensions, to directly infer the diffusion coefficient of a nanoparticle without calculating a mean-square displacement, and to quantify the ejection of DNA from an individual lambda phage virus, demonstrating that the approach can be used to infer both static and dynamic properties of nanoscale systems. | result 0.63 | 0.96 | 0.82 | asserted 0.99 |
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Original abstract
Interferometric scattering microscopy (iSCAT) can image the dynamics of nanometer-scale systems. The typical approach to analyzing interferometric images involves intensive processing, which discards data and limits the precision of measurements. We demonstrate an alternative approach: modeling the interferometric point spread function (iPSF) and fitting this model to data within a Bayesian framework. This approach yields best-fit parameters, including the particle's three-dimensional position and polarizability, as well as uncertainties and correlations between these parameters. Building on recent work, we develop a model that is parameterized for rapid fitting. The model is designed to work with Hamiltonian Monte Carlo techniques that leverage automatic differentiation. We validate this approach by fitting the model to interferometric images of colloidal nanoparticles. We apply the method to track a diffusing particle in three dimensions, to directly infer the diffusion coefficient of a nanoparticle without calculating a mean-square displacement, and to quantify the ejection of DNA from an individual lambda phage virus, demonstrating that the approach can be used to infer both static and dynamic properties of nanoscale systems.
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