Constraints on mass matrices due to measured property of the mixing matrix

It is shown that two specific properties of the unitary matrix $V$ can be expressed directly in terms of the matrix elements and eigenvalues of the hermitian matrix $M$ which is diagonalized by $V$. These are the asymmetry $\Delta(V)= |V_{12}|^2- |V_{21}|^2$, of $V$ with respect to the main diagonal and the Jarlskog invariant $J(V)= {\rm Im}(V_{11}V_{12}^* V_{21}^* V_{22})$. These expressions for $\Delta(V)$ and $J(V)$ provide constraints on possible mass matrices from the available data on $V$.