Pith Number

pith:3C6YFQMS

pith:2026:3C6YFQMS2UV35UDJKUN4TTOAWT

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Chem-SIM: Super-resolution Chemical Imaging via Photothermal Modulation of Structured-Illumination Fluorescence

Biwen Gao, Danchen Jia, Dashan Dong, George Abu-Aqil, Jianpeng Ao, Ji-Xin Cheng, Meng Zhang, Qing Xia, Xinyan Teng

Chem-SIM recovers full vibrational fingerprints at SIM-grade resolution by photothermal modulation of structured-illumination fluorescence.

arxiv:2602.16079 v2 · 2026-02-17 · physics.optics

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2 Internet Archive

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Claims

C1strongest claim

Chem-SIM preserves full vibrational fingerprints, achieves SIM-grade lateral resolution in a high-throughput camera-based format, and distinguishes stationary- from log-phase bacteria through chemical content mapping while reporting deuterated fatty-acid incorporation and lipid-droplet dynamics in live cells.

C2weakest assumption

The assumption that the weak IR-induced fluorescence intensity change can be accurately recovered from low-photon-budget data via Poisson maximum-likelihood demodulation and spectral normalization without introducing artifacts that distort the chemical fingerprints.

C3one line summary

Chem-SIM achieves super-resolved chemical imaging of cells by demodulating photothermal modulation of structured-illumination fluorescence to extract vibrational spectra.

References

47 extracted · 47 resolved · 1 Pith anchors

[1] Valm, A.M. et al. Applying systems -level spectral imaging and analysis to reveal the organelle interactome. Nature 546, 162-167 (2017) 2017

[2] Bar-Peled, L. & Kory, N. Principles and functions of metabolic compartmentalization. Nature Metabolism 4, 1232-1244 (2022). 14 2022

[3] Huang, B., Bates, M. & Zhuang, X. Super -Resolution Fluorescence Microscopy. Annual Review of Biochemistry 78, 993-1016 (2009) 2009

[4] Surpassing the late ral resolution limit by a factor of two using structured illumination microscopy 2000

[5] Wu, Y. & Shroff, H. Faster, sharper, and deeper: structured illumination microscopy for biological imaging. Nature Methods 15, 1011-1019 (2018) 2018

Formal links

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Receipt and verification

First computed	2026-05-17T23:39:16.094949Z
Builder	pith-number-builder-2026-05-17-v1
Signature	Pith Ed25519 (`pith-v1-2026-05`) · public key
Schema	pith-number/v1.0

Canonical hash

d8bd82c192d52bbed069551bc9cdc0b4e0a75bfc3c38ca86e035b5ff05c1033a

Aliases

arxiv: 2602.16079 · arxiv_version: 2602.16079v2 · doi: 10.48550/arxiv.2602.16079 · pith_short_12: 3C6YFQMS2UV3 · pith_short_16: 3C6YFQMS2UV35UDJ · pith_short_8: 3C6YFQMS

Agent API

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Verify this Pith Number yourself

curl -sH 'Accept: application/ld+json' https://pith.science/pith/3C6YFQMS2UV35UDJKUN4TTOAWT \
  | jq -c '.canonical_record' \
  | python3 -c "import sys,json,hashlib; b=json.dumps(json.loads(sys.stdin.read()), sort_keys=True, separators=(',',':'), ensure_ascii=False).encode(); print(hashlib.sha256(b).hexdigest())"
# expect: d8bd82c192d52bbed069551bc9cdc0b4e0a75bfc3c38ca86e035b5ff05c1033a

Canonical record JSON

{
  "metadata": {
    "abstract_canon_sha256": "3383d281d8606ec7d5bf84d1dabb14023ee93a9cb528c2a79db2b8da60127a12",
    "cross_cats_sorted": [],
    "license": "http://creativecommons.org/licenses/by-nc-nd/4.0/",
    "primary_cat": "physics.optics",
    "submitted_at": "2026-02-17T23:11:23Z",
    "title_canon_sha256": "0a86b6cc8400cc1a441ae33e03382d6c209d1955cddae5160b4955d964ee9f7a"
  },
  "schema_version": "1.0",
  "source": {
    "id": "2602.16079",
    "kind": "arxiv",
    "version": 2
  }
}