Fast fluorescence dynamics in non-ratiometric calcium indicators
classification
⚛️ physics.bio-ph
cond-mat.soft
keywords
fluorescencenon-ratiometriccalciumdecaydynamicsindicatorsogb-1accurate
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A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.
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